Effect of pleurotus sajor-caju polysaccharide encapsulated in poly D, L lactide-co-glycolide nanoparticles for HPV vaccine in murine model

Evelyn Piyachat, Roopngam and Evelyn Roopngam, Piyachat (2018) Effect of pleurotus sajor-caju polysaccharide encapsulated in poly D, L lactide-co-glycolide nanoparticles for HPV vaccine in murine model. Nanomedicine Journal, 5 (3). pp. 144-151.

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Abstract

Objective(s): In the current work, poly D, L lactide-co-glycolide (PLGA) particles were applied for a viral vaccine for the delivery of antigens in cytosolic pathway by increasing the antigen presentation to T-lymphocytes. HPV-E7 protein with PLGA particles has been reported as a potent adjuvant for HPV vaccine by encapsulating protein into the PLGA particles. Polysaccharide from Pleurotus sajor-caju was also applied as a potent immunomodulator. Materials and Methods: HPV-E7 protein and Pleurotus sajor-caju polysaccharides (PSC) were encapsulated into PLGA nanoparticles. This combination comprised a strategy to induce helper and cytotoxic T-lymphocytes (CTL) expansion. Mice antibodies and T-lymphocyte expansion were investigated in comparison between encapsulated E7 protein into PLGA nanoparticles (E7PLGA) and E7 protein with PSC encapsulated into PLGA nanoparticles (PSC-E7PLGA). Results: The results showed that E7PLGA and PSC-E7PLGA could induce antibody response to HPV. The PSC-E7PLGA could increase the level of viral antigen-specific IgG antibodies. The cellular immune responses are also significantly enhanced by expansion of helper T-lymphocytes and CTL. PSC-E7PLGA was shown to be significantly higher in immunomodulating activity than E7PLGA. Conclusion: Thus, the encapsulation of polysaccharide and HPV-E7 antigen into PLGA nanoparticles is a strategy for development of HPV vaccine.

Item Type: Article
Subjects: QT physiology
Divisions: Journals > Nanomedicine Journal
Depositing User: nmj nmj
Date Deposited: 22 Jul 2018 05:45
Last Modified: 22 Jul 2018 05:45
URI: http://eprints.mums.ac.ir/id/eprint/10203

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