Targeted detection of the cancer cells using the anti-CD24 bio modified PEGylated gold nanoparticles: the application of CD24 as a vital cancer biomarker

Fazel-Ghaziyani, Mona and Shahbazi-Gahrouei, Daryoush and Pourhassan-Moghaddam, Mohammad and Baradaran, Behzad and Ghavami, Mostafa (2018) Targeted detection of the cancer cells using the anti-CD24 bio modified PEGylated gold nanoparticles: the application of CD24 as a vital cancer biomarker. Nanomedicine Journal, 5 (3). pp. 172-179.

[img] Text
NMJ_Volume 5_Issue 3_Pages 172-179.pdf

Download (521kB)
Official URL: http://nmj.mums.ac.ir/article_11164.html

Abstract

Objective(s): The central role of molecular imaging modalities in cancer management is an undeniable fact that could help to diagnose cancer tumors in early stages. The main aim of this study is to prepare a novel targeted molecular imaging nanoprobe of CD24-PEGylated Au NPs to improve the ability of Computed tomography scanning (CT scan) outputs for both in vitro and in vivo detection of breast cancer (4T1) cells. Materials and methods: Gold nanoparticles (Au NPs) were synthesized and coated with polyethylene glycol (PEG) chains in order to improve the stability of the Au NPs and to provide bio modification points for antibody immobilization. The synthesized nanoprobe was used for both in vitro and in vivo targeted CT imaging breast cancer cells (4T1) and the xenograft tumor model. Results: Findings showed that the attenuation coefficient of 4T1 cells that were targeted by CD24-PEGylated Au NPs is calculated to be over two times higher than the untargeted 4T1 cells (15 HU vs 39 HU, respectively). Indeed, the results clearly reveal that the developed CD24-PEGylated Au NPs showed the tumor CT enhancement was higher than that of Omnipaqe which used as control. Also, the CT number values of the tumor area at different time points gradually increased after 60 min post injection and was significantly higher than before injection. Conclusions: Results showed the introduced CT imaging nanoprobe (Au NPs-PEGylated) could be useful for CT imaging of breast tumors under in vivo condition. Overall, it is concluded that Au NPs-PEGylated contrast media is able to detect breast cancer (4T1) cells and is more effective compared with other casual methods.

Item Type: Article
Subjects: QT physiology
Divisions: Journals > Nanomedicine Journal
Depositing User: nmj nmj
Date Deposited: 22 Jul 2018 05:49
Last Modified: 22 Jul 2018 05:49
URI: http://eprints.mums.ac.ir/id/eprint/10206

Actions (login required)

View Item View Item