Fetal microchimerism in mouse caerulein-induced pancreatitis model

Vojdani, Zahra and Bagheri, Jafar and Talaei-Khozani, Tahereh and Azarpira, Negar and Salmannjad, Mahin and Farokhi, Ali (2018) Fetal microchimerism in mouse caerulein-induced pancreatitis model. Iranian Journal of Basic Medical Sciences, 21 (9). pp. 889-895.

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Abstract

Objective(s): Fetal microchimerism is the persistence of allogeneic cell population that transfer from the fetus to the mother. The aim of this study was to evaluate the presence of fetal microchimerism in the pancreas of the mouse with acute pancreatitis (AP).Materials and Methods: In this experimental study, female wild-type mice were mated with male EGFP+. AP model was obtained by injection of caerulein two days after delivery. Sixty mice were divided into 3 groups: the virgin pancreatitis-induced animals, pregnant pancreatitis-induced animals mated with transgenic EGFP mice, and pregnant sham animals. To prove pancreatitis induction, the blood amylase and lipase were assessed; and pancreas was removed from a subpopulation of each group for histopathological examinations after 6 hr. The remaining mice were kept for 3 weeks and histopathological exanimation, immunohistochemistry, and PCR were performed.Results: EGFP+ cells were found in acini and around the blood vessels in the pancreas of pregnant pancreatitis-induced animals. They differentiated to acinar, adipocyte-like, and mesenchymal-like cells. PCR showed that 20 of the pregnant pancreatitis-induced animals were EGFP+. The histopathological study showed improvement in pancreatitis scores in the mice with history of pregnancy. Conclusion: It seems that pregnancy has a beneficial impact on caerulein-induced pancreatitis and improves the pancreatitis score in mouse.

Item Type: Article
Subjects: QT physiology
Divisions: Journals > Iranian J Basic Medical Sciences
Depositing User: ijbms ijbms
Date Deposited: 01 Oct 2018 09:18
Last Modified: 01 Oct 2018 09:18
URI: http://eprints.mums.ac.ir/id/eprint/10353

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