Mangosteen peel extract (Garcinia mangostana L.) as protective agent in glucose-induced mesangial cell as in vitro model of diabetic glomerulosclerosis

Widowati, Wahyu and Laksmitawati, Dian and Wargasetia, Teresa and Afifah, Ervi and Amalia, Annisa and Arinta, Yukko and Rizal, Rizal and Suciati, Tri (2018) Mangosteen peel extract (Garcinia mangostana L.) as protective agent in glucose-induced mesangial cell as in vitro model of diabetic glomerulosclerosis. Iranian Journal of Basic Medical Sciences, 21 (9). pp. 972-977.

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Abstract

Objective(s): This study aims to evaluate the activity of mangosteen peels extract (MPE) as protection agent on induced-glucose mesangial cells (SV40 MES 13 cell line (Glomerular Mesangial Kidney, Mus Musculus)). Materials and Methods: MPE was performed based on maceration method. Cytotoxic assay was performed based on MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) method, while the level of TGF-β1 (Transforming growth factor-β1) and fibronectin in glucose-induced mesangial cells were assayed and determined using ELISA KIT. Results: In viability assay, MPE 5 and 20 µg/ml has the highest activity to increase cells proliferation in glucose-induced mesangial cells at 5, 10, and 15 days of incubation in glucose concentration (5 and 25 mM) (P<0.05). In inhibitory activity of TGF-β1 and fibronectin level, MPE 5 µg/ml (glucose-induced 5 mM) show the lowest level compared to positive control and other treatments (P<0.05).Conclusion: MPE can increase cell proliferation in glucose-induced mesangial cells and significantly reduce the level of TGF-β1 and fibronectin. MPE activity has correlates to inhibit the diabetic glomerulosclerosis condition and may increase mesangial cell proliferation.

Item Type: Article
Subjects: QV pharmacology
Depositing User: ijbms ijbms
Date Deposited: 02 Oct 2018 05:37
Last Modified: 02 Oct 2018 05:37
URI: http://eprints.mums.ac.ir/id/eprint/10364

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