Protective role of Lactobacillus plantarum A7 against irinotecan-induced genotoxicity

Sepahi, Soheila and Jafarian-Dehkordi, Abbas and Mirlohi, Maryam and shirani, kobra and Etebari, Mahmoud (2016) Protective role of Lactobacillus plantarum A7 against irinotecan-induced genotoxicity. Avicenna Journal of Phytomedicine, 6 (3). pp. 329-335.

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Abstract

Objective: Irinotecan is a botanical derivative and an anti-cancer drug with cytotoxic and genotoxic effects. The present study evaluated the effect of Lactobacillus plantarum A7 on the genotoxic activity of irinotecan in a hepatocellular carcinoma cell line (HepG2) by comet assay. Materials and Methods: HepG2 were incubated with irinotecan (100 µM), heat-killed cells (0.025 µg/ml) + irinotecan (100 µM), and cell-free supernatants (0.5 and 1 µg/ml) of L. plantarum A7 + irinotecan (100 µM). Phosphate buffered saline (PBS) was used as negative control. Results: Irinotecan was shown to induce DNA damage in HepG2 cells. The results showed that heat-killed cells (0.025 µg/ml) and cell-free supernatants (0.5 and 1 µg/ml) of L. plantarum significantly reduce irinotecan- induced DNA damage. Conclusion: Our results indicate that L. plantarum A7 can decrease the genotoxic effects of irinotecan in HepG2 cells, in vitro. This finding may be supportive for the optimization of therapeutic efficacy in irinotecan treatment.

Item Type: Article
Subjects: QV pharmacology
Divisions: Journals > Avicenna J Phytomedicine
Depositing User: ajp ajp
Date Deposited: 19 Sep 2017 17:10
Last Modified: 19 Sep 2017 17:10
URI: http://eprints.mums.ac.ir/id/eprint/1042

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