Comparison of the Effect of Tissue Inhibitors on the Antibacterial Efficacy of a New Silver Nanoparticle Irrigant Containing Sodium Hypochlorite and Chlorhexidine

Adl, Alireza and Motamedifar, Mohammad and Sobh Namayan, Fereshteh and Shahcheraghi, Ladan and Vossoughi, Mehrdad and Gholami, Ahmad (2018) Comparison of the Effect of Tissue Inhibitors on the Antibacterial Efficacy of a New Silver Nanoparticle Irrigant Containing Sodium Hypochlorite and Chlorhexidine. Journal of Mashhad Dental School, 42 (4). pp. 320-328.

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Abstract

Introduction: The present study aimed to evaluate the effects of various tissue inhibitors, including dentin powder, dentin matrix, bovine serum albumin, and bacterial lipopolysaccharide, on the antibacterial activity of sodium hypochlorite (NaOCl), chlorhexidine (CHX), and a new silver nanoparticle irrigant (Im-based AgNPs). Materials and Methods: In the first set of experiments, 950 µL of each irrigating solution was mixed with 50 µL of an Enterococcus faecalis suspension, and bacterial survival was assessed after 10, 30, and 60 seconds. In the second set of experiments, 100 µL of each inhibitor suspension was mixed with 100 µL of each irrigating solution and incubated for one hour. Afterwards, 100 µL of the bacterial suspension was added. Bacterial survival was assessed after 10 seconds and 10 and 60 minutes. Both sets of experiments were performed in triplicate. The colony-forming units were counted, and the log-transformed number was analyzed using the Kruskal-Wallis and Dunn tests. In the statistical analysis, P-value of less than 0.05 was considered significant. Results: In the first set of the experiments, the antibacterial activity of NaOCl and CHX was significantly higher compared to the AgNPs and saline solutions at all the times (P˂0.05). In the second set of the experiments, the four inhibitors significantly inhibited the antibacterial effects of CHX and NaOCl (P˂0.05), with the exception of bacterial lipopolysaccharide, which had no effect on NaOCl. Similar to the negative control, AgNPs showed the high survival of the bacteria regardless of the presence or absence of the tissue inhibitors. Conclusion: According to the results, the tissue inhibitors affected the antibacterial activity of NaOCl and CHX at variables degrees. However, AgNPs showed no antibacterial activity regardless of the presence or absence of the inhibitors.

Item Type: Article
Subjects: WU Dentistry. Oral surgery
Divisions: Journals > Journal of Mashhad Dental School
Depositing User: jmds jmds
Date Deposited: 25 Dec 2018 09:41
Last Modified: 25 Dec 2018 09:41
URI: http://eprints.mums.ac.ir/id/eprint/10685

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