Antioxidant effects of gold nanoparticles on Schistosoma mansoni induced granuloma, in vitro

El-Shorbagy, Ayatollah and Gamil, Irene S and Mohey, Mohammed A and Nady, Soad (2019) Antioxidant effects of gold nanoparticles on Schistosoma mansoni induced granuloma, in vitro. Nanomedicine Journal, 6 (1). pp. 19-26.

[img] Text
NMJ_Volume 6_Issue 1_Pages 19-26.pdf

Download (333kB)
Official URL: http://nmj.mums.ac.ir/article_12154.html

Abstract

Objective(s): Schistosomiasis and hepatitis C virus HCV co-infection is common among the Egyptian population. Co-infected patients have higher rate of chronic hepatitis, cirrhosis and hepatocellular carcinoma. The aim of the present study was to investigate the potential role of gold nanoparticles on granuloma in vitro. Materials and Methods: In the current study, granulocytes were isolated from the blood of 50 Schistosoma/HCV co-infected patients and 25 healthy subjects. Granulocytes were used to induce granuloma in vitro in the presence of polyacrylamide beads coated with Schistosoma mansoni soluble egg antigen and interleukin-17. In addition, granuloma was treated on the 3rd day with gold nanoparticles alone or in the presence of human hepatocellular carcinoma cell line (Hep G2) as carcinoma model. Praziquantel (PZQ) was used as a positive control. Granuloma index was determined on the 7th and 14th day. Furthermore, the supernatants were collected to measure the granulocyte mediators including tumor necrosis factor alpha TNF-α, hydrogen peroxide H2O2 and nitric oxide NO by ELISA on the 7th and 14th day. Results: Treatment with AuNPs in the presence of Hep G2 showed a significant reduction in granuloma index and granulocyte mediators including H2O2 and NO, while a significant elevation was observed in TNF-α level as compared to their corresponding values in the presence of IL-17 in both healthy individual and co-infected patients on the 7th and 14th day. Conclusion: In conclusion, the presence of IL-17 accelerated the formation of granuloma and the treatment with AuNPs in the presence Hep G2 cells indicated that AuNPs were more effective antioxidant agents than PZQ.

Item Type: Article
Subjects: QT physiology
Divisions: Journals > Nanomedicine Journal
Depositing User: nmj nmj
Date Deposited: 23 Jan 2019 05:26
Last Modified: 23 Jan 2019 05:26
URI: http://eprints.mums.ac.ir/id/eprint/11054

Actions (login required)

View Item View Item