Evaluation of lentinan effects on cytochrome P450 activity in rats by a cocktail method

Lin, Yiping and Wei, Yanli and Hu, Xiaoxia and Wu, Mei-Ling and Yao, Jingchan and Ying, Xiaoqian and Fu, Xiaoyan and Ding, Mingxing and Qiao, Liman (2019) Evaluation of lentinan effects on cytochrome P450 activity in rats by a cocktail method. Iranian Journal of Basic Medical Sciences, 22 (3). pp. 296-301.

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Abstract

Objective(s): In this study, a cocktail of probe drugs was used to assess whether lentinan could influence the activities of rat enzymes CYP3A4, CYP2D6, CYP1A2, CYP2C19, and CYP2C9 in vivo. Materials and Methods: Fourteen days after intraperitoneal injection of lentinan, rats were given an oral dose of a cocktail solution containing phenacetin, tolbutamide, omeprazole, metoprolol, and midazolam. Then, we obtained blood in specific durations for the determination of plasma concentration of the probe drugs using UPLC-MS/MS. We also evaluated the pharmacokinetic parameters using the DAS 2.0 software. Results: We found that various concentrations of lentinan increased the activity of rat CYP1A2, CYP3A4, CYP2D6, and CYP2C19 but not CYP2C9. Conclusion: These findings suggest that clinical application of lentinan combination with CYP3A4, CYP1A2, CYP2C19, or CYP2D6 should be given careful consideration as this may lead to herb-drug interactions and hence treatment failure.

Item Type: Article
Subjects: QV pharmacology
Divisions: Journals > Iranian J Basic Medical Sciences
Depositing User: ijbms ijbms
Date Deposited: 10 Jun 2019 07:54
Last Modified: 10 Jun 2019 07:54
URI: http://eprints.mums.ac.ir/id/eprint/11279

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