Cytotoxic effects of auraptene against a human malignant glioblastoma cell line

Afshari, Amir R. and Karimi Roshan, Mostafa and Soukhtanloo, Mohammad and Ghorbani, Ahmad and Rahmani, Farzad and Jalili-nik, Mohammad and Vahedi, Mohammad Mahdi and Hoseini, Azar and Sadeghnia, Hamid R. and Mollazadeh, Hamid and Mousavi, Seyed Hadi (2019) Cytotoxic effects of auraptene against a human malignant glioblastoma cell line. Avicenna Journal of Phytomedicine, 9 (4). pp. 334-346.

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Abstract

Objective: Glioblastoma multiforme (GBM) is the deadliest type of primary brain tumors, and the survival of patients is estimated to be only about one year. This study, for the first time, investigated the cytotoxic effects of auraptene on U87 GBM cell line. Materials and Methods: The cellular toxicity was measured by the MTT assay following 24 and 48-hr treatment with different concentrations of auraptene (0-400μg/ml). Apoptosis was evaluated by sub-G1 peak in cell cycle analysis of propidium-iodide- stained nuclei. Moreover, to determine the Bax, Bcl-2, MCP-1, NF-κB, IL-1β, and p53 genes expression, we used real-time polymerase chain reaction (RT-PCR). Results: The results revealed that auraptene reduced the viability of U87 cells concentration- and time-dependently with IC50 values of 108.9 and 79.17μg/ml obtained for 24 and 48-hr treatments, respectively. Also, sub-G1 population was significantly increased following 24 (p real-time RT-PCR showed an up-regulation in Bax, NF-κB, IL-1β, and p53 but a down-regulation in MCP-1 and Bcl-2 genes expression. Conclusion: This study showed that auraptene triggered apoptosis probably through Bax/Bcl-2 regulation, blocked cell cycle progression and inhibited proliferation in U87 GBM cells. Taken together, auraptene can be utilized as an effective natural medicine against GBM, after complementary studies.

Item Type: Article
Subjects: QT physiology
WK Endocrine System
WL Nervous system
QV pharmacology
Divisions: Journals > Avicenna J Phytomedicine
Depositing User: ajp ajp
Date Deposited: 01 Jul 2019 05:55
Last Modified: 01 Jul 2019 05:55
URI: http://eprints.mums.ac.ir/id/eprint/11729

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