Inhibitory Effect of Nano-gel/Emulsion of Chitosan Coating Incorporated with Ziziphora Clinopodioides Essential Oil and Nisin on Escherichia Coli O157:H7 Inoculated in Beef at Cold Storage Condition

Azizian, Asghar and Khanzadi, Saeid and Hashemi, Mohammad and Azizzadeh, Mohammad (2019) Inhibitory Effect of Nano-gel/Emulsion of Chitosan Coating Incorporated with Ziziphora Clinopodioides Essential Oil and Nisin on Escherichia Coli O157:H7 Inoculated in Beef at Cold Storage Condition. Journal of Nutrition,Fasting and Health, 7 (2). pp. 103-109.

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Abstract

Introduction: This study was conducted to evaluate the influence of chitosan Nano-gel/emulsion coating functionalized by Ziziphora clinopodioides essential oil (ZCEO) and nisin on growth inhibition of Escherichia coli O157: H7 inoculated in beef samples during 16 days in cold storage condition (4�C). Methods: Beef sample was divided into six groups after inoculation of E. coli O157:H7. Treatments including control (no coating), chitosan 2, sonicated chitosan 2, Nano-emulsion of chitosan coating containing ZCEO (0.5), Nano-gel of chitosan coating containing nisin (200 IU/g), Nano-emulsion of chitosan coating containingZCEO (0.5) and nisin (200 IU/g) were stored at refrigeration temperature and bacterial count were performed on days: 0, 1, 2, 4, 8, 12 and 16. Data were analyzed using repeated measure ANOVA and Bonferroni post hoc tests. Results: Result indicated a significant reduction in E. coli O157:H7 count in all treatments when compared to control group and the highest inhibitory activity was observed in chitosan Nano-emulsion coating containing ZCEO (0.5) and nisin (200 IU/g). Conclusion: Accordingly, it is suggested that chitosan Nano-emulsion coating with ZCEO and nisinpractically be applied in beef to increase its safety against pathogenic bacteria especially E. coli O157: H7.

Item Type: Article
Uncontrolled Keywords: Chitosan,Functional coating,Nano technology,Beef,Escherichia coli O157:H7
Subjects: QU Biochemistry
Divisions: Journals > J Fasting And Health
Depositing User: Journal of Nutriton Fasting and Health
Date Deposited: 15 Jul 2019 05:54
Last Modified: 15 Jul 2019 05:54
URI: http://eprints.mums.ac.ir/id/eprint/11833

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