Myelin enhancement of Multiple sclerosis model with gold nanoparticles into the corpus callosum

Khosravi, Mahjabin and Karami, Manizheh and Jalali Nadoushan, Mohammadreza and Hajnorouzi, Abazar (2019) Myelin enhancement of Multiple sclerosis model with gold nanoparticles into the corpus callosum. Nanomedicine Journal, 6 (4). pp. 321-328.

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Abstract

Objective(s): With no substantial cost, we injected L-arginine into the rat’s corpus callosum (CC) to create animal model of multiple sclerosis (MS) and investigated the pre-injection effect of gold nanoparticles (GNPs). Materials and Methods: Adult male Wistar rat (250-300 g) was surgically cannulated at the CC, and after recovery it was injected L-arginine (3-200 µg/rat, intra-CC) once daily for 3 to 5 consecutive days. GNPs (0.001-0.01 µg/rat, intra-CC) were injected alone or prior to the L-arginine using the same procedure. Control group solely received saline (1 µL/rat, intra-CC). Brain was studied with luxol fast blue. Weight change was also analyzed via the analysis of variance (ANOVA). Results: L-arginine significantly induced (p< 0.05) a reduction in the fiber density while the neurons increased (p< 0.05). Single GNPs reduced (p< 0.05) the fiber and neuron densities; however, pre-injection of NPs caused myelinated fibers and uniform density of neurons. Conclusion: The L-arginine may trigger demyelination by pro-inflammatory nitric oxide (NO), and the GNPs may improve this effect.

Item Type: Article
Uncontrolled Keywords: Corpus callosum,Demyelination,Gold Nanoparticle,L-arginine,Multiple Sclerosis,Nitric oxide,Rat
Subjects: QT physiology
Divisions: Journals > Nanomedicine Journal
Depositing User: nmj nmj
Date Deposited: 22 Dec 2019 10:22
Last Modified: 22 Dec 2019 10:22
URI: http://eprints.mums.ac.ir/id/eprint/12324

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