Cytotoxicity of orthodontic bonding adhesive resins on human oral fibroblasts

Ahrari, F. and Tavakkol Afshari, J. and Poosti, M. and Brook, A. (2010) Cytotoxicity of orthodontic bonding adhesive resins on human oral fibroblasts. European Journal of Orthodontics, 32 (6). pp. 688-692.

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There is little information concerning the cytotoxic effects of no-mix and flowable adhesives used in orthodontics. The aim of the present study was to evaluate the cytotoxic effects of a no-mix (Unite), a light-cured (Tranbond XT), and a flowable (Denfil Flow) adhesives on human oral fibroblasts.Twelve discs of each adhesive were prepared and aged for 1, 3, 5, and 7 days in Dulbecco's Modified Eagle's Medium (DMEM). Cell viability was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and the difference between the groups was tested by analysis of variance and Tukey tests (α = 0.05).After 1 day of storage, the no-mix adhesive showed moderate cytotoxic effects (P < 0.05), while the light-cured and flowable adhesives were essentially non-cytotoxic. Ageing considerably reduced the cytotoxicity of the no-mix adhesive. On days 5 and 7 of the experiment, the cell viability of three adhesives did not differ significantly (P > 0.05), but cell viability was slightly reduced on day 7.Moderate cytotoxic effects of no-mix adhesive on the first day of the experiment suggest that care should be taken to protect dentists and patients when these adhesives are being handled. Despite higher resin components, the flowable adhesive showed excellent biocompatibility. © The Author 2010. Published by Oxford University Press on behalf of the European Orthodontic Society.

Item Type: Article
Additional Information: Cited By :20 Export Date: 16 February 2020 CODEN: EJOOD Correspondence Address: Poosti, M.; Department of Orthodontics, Faculty of Dentistry, Mashhad University of Medical Sciences, Vakilabad Boulevard, Mashhad, Iran; email:
Uncontrolled Keywords: acrylic acid resin Denfil Flow resin cement Transbond XT Unite dental cement analysis of variance article cell culture cell survival cytology dental bonding drug effect fibroblast gingiva human nonparametric test orthodontic device time Acrylic Resins Cells, Cultured Fibroblasts Humans Orthodontic Brackets Resin Cements Statistics, Nonparametric Time Factors
Subjects: WU Dentistry. Oral surgery
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib4 lib4
Date Deposited: 03 Mar 2020 09:11
Last Modified: 03 Mar 2020 09:11

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