AS1411 Aptamer-Decorated Biodegradable Polyethylene Glycol-Poly(lactic-co-glycolic acid) Nanopolymersomes for the Targeted Delivery of Gemcitabine to Non-Small Cell Lung Cancer in Vitro

Alibolandi, M. and Ramezani, M. and Abnous, K. and Hadizadeh, F. (2016) AS1411 Aptamer-Decorated Biodegradable Polyethylene Glycol-Poly(lactic-co-glycolic acid) Nanopolymersomes for the Targeted Delivery of Gemcitabine to Non-Small Cell Lung Cancer in Vitro. Journal of Pharmaceutical Sciences, 105 (5). pp. 1741-1750.

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Abstract

Molecularly targeted drug delivery systems represent a novel therapeutic strategy in the treatment of different cancers. In the present study, we have developed gemcitabine (GEM)-loaded AS1411 aptamer surface-decorated polyethylene glycol-poly(lactic-co-glycolic acid) nanopolymersome (Apt-GEM-NP) to target nucleolin-overexpressing non-small cell lung cancer (NSCLC; A549). The prepared Apt-GEM-NP showed average particle size of 128 ± 5.23 nm and spherical morphology with encapsulation efficiency and loading content of 95.32 ± 2.37 and 8.61 ± 0.27, respectively. Apt-GEM-NP exhibited a controlled release pattern. A sustained release of drug in physiological conditions will greatly improve the chemotherapeutic efficiency of a system. Enhanced cellular uptake and the cytotoxicity of aptamer-conjugated nanoparticles (NPs) in A549 cancer cells obviously verified nucleolin-mediated receptor-based active targeting. Nucleolin-mediated internalization of the targeted polymeric NP was further confirmed by flow cytometry and fluorescence microscopy. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay clearly showed the enhanced cell proliferation inhibitory effect of AS1411-conjugated NP on account of the selective delivery of GEM to the nucleolin-overexpressing cancer cells. Our results showed that AS1411 aptamer conjugation on the surface of NP could be a potential treatment strategy for A549 as a nucleolin-overexpressing cell line. This suggests that AS1411-GEM-NPs could be potentially used for the treatment of NSCLC. © 2016 American Pharmacists Association® Published by Elsevier Inc. All rights reserved.

Item Type: Article
Additional Information: Cited By :38 Export Date: 16 February 2020 CODEN: JPMSA Correspondence Address: Abnous, K.; Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical SciencesIran; email: abnouskh@mums.ac.ir
Uncontrolled Keywords: cancer chemotherapy drug delivery system encapsulation nanoparticle PLGA polymeric drug delivery systems targeted drug delivery aptamer gemcitabine nanopolymersome nucleolin polyglactin unclassified drug AGRO 100 antineoplastic antimetabolite deoxycytidine macrogol derivative oligodeoxyribonucleotide polyester polyethylene glycol-poly(lactide-co-glycolide) polymer A549 cell line antiproliferative activity Article biodegradation cell proliferation cell transport controlled drug release controlled study covalent bond cytotoxicity drug conjugation feasibility study flow cytometry fluorescence microscopy gene overexpression hydrophilicity hydrophobicity in vitro study internalization molecular weight molecularly targeted therapy MTT assay nanoencapsulation non small cell lung cancer particle size protein targeting surface property sustained drug release zeta potential A-549 cell line analogs and derivatives animal CHO cell line Cricetulus hamster human lung tumor metabolism procedures tumor cell line A549 Cells Animals Antimetabolites, Antineoplastic Carcinoma, Non-Small-Cell Lung Cell Line, Tumor CHO Cells Cricetinae Drug Delivery Systems Humans Lung Neoplasms Nanoparticles Oligodeoxyribonucleotides Polyesters Polyethylene Glycols Polymers
Subjects: WF Respiratory System
QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib4 lib4
Date Deposited: 03 Mar 2020 07:05
Last Modified: 03 Mar 2020 07:05
URI: http://eprints.mums.ac.ir/id/eprint/12912

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