Growth inhibition and apoptosis induction of essential oils and extracts of Nepeta cataria L. on human prostatic and breast cancer cell lines

Emami, S. A. and Asili, J. and HosseinNia, S. and Yazdian-Robati, R. and Sahranavard, M. and Tayarani-Najaran, Z. (2016) Growth inhibition and apoptosis induction of essential oils and extracts of Nepeta cataria L. on human prostatic and breast cancer cell lines. Asian Pacific Journal of Cancer Prevention, 17. pp. 125-130.

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Nepeta cataria L. has been used in traditional medicine of some countries. Here the cytotoxic and apoptogenic activity of methanol extracts, n-hexane, dichloromethane, ethyl acetate, n-butanol, and acqueous extracts and the essential oil obtained from the aerial parts of the plant were evaluated with PC3, DU-145 and MCF-7 cell lines. Cell viability, histograms of PI stained fragmented DNA in apoptotic cells and Western blot analysis of proteins involved in the cascade of apoptosis were compared in all samples. Thirty components were identified as volatile, representing 99.7 of essential oil composition after GC-MS analysis of the oil obtained from aerial parts of the N. cataria by hydrodistillation. The major oil components of the essential oil were nepetalactone stereoisomers. Comparing IC50 values showed estrogen receptor positive PC3 cells were more sensitive to the cytotoxic effects of N. cataria in comparison with low hormone-receptor presenting DU-145 cells. Among multiple extracts and essential oils of the plant, only the ethyl acetate extract could significantly decrease cell viability in PC3 cells, in a concentration dependent manner. Ethyl acetate extract of N. cataria treated cells showed a sub-G1 peak in PC3 cells in a concentration dependent manner that indicates the involvement of an apoptotic process in ethyl acetate extract-induced cell death. Western blotting analysis showed that in PC3 cells treated with ethyl acetate (48 h) caspase 3 and PARP were cleaved to active forms. Overall, the results suggest that further analytical elucidation of N. cataria in respect to finding new cytotoxic chemicals with anti-tumor activity is warranted. © 2016, Asian Pacific Journal of Cancer Prevention.

Item Type: Article
Additional Information: Cited By :9 Export Date: 16 February 2020 Correspondence Address: Tayarani-Najaran, Z.; Department of Pharmacodynamics and Toxicology, Mashhad University of Medical SciencesIran; email:
Uncontrolled Keywords: Apoptosis Cytotoxic Essential oil Lamiaceae Nepeta cataria L plant extract vegetable oil breast tumor cell proliferation chemistry drug effects female flow cytometry human male mass fragmentography Nepeta pathology phytotherapy Prostatic Neoplasms tumor cell culture Western blotting Blotting, Western Breast Neoplasms Gas Chromatography-Mass Spectrometry Humans Oils, Volatile Plant Extracts Plant Oils Tumor Cells, Cultured
Subjects: QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib4 lib4
Date Deposited: 02 Mar 2020 10:34
Last Modified: 02 Mar 2020 10:34

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