Extraction and purification of crocin from saffron stigmas employing a simple and efficient crystallization method

Hadizadeh, F. and Mohajeri, S. A. and Seifi, M. (2010) Extraction and purification of crocin from saffron stigmas employing a simple and efficient crystallization method. Pakistan Journal of Biological Sciences, 13 (14). pp. 691-698.

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Abstract

Abstract: In this study, total crocin was extracted from saffron stigmas using crystallization method. Ethanol 80 was selected as the best extraction solvent. Crystallization process was carried out in one and two steps at different temperatures. Ethanol 80 was used as crystallization medium. Crocin crystals obtained from the first crystallization had low purity and thus were subjected to the second crystallization. The higher purity crystals were yielded in the second crystallization at -5°C. The purity of crocin crystals was studied using UV-visible spectrophotometery and HPLC in comparison with Fluka product and methanolic extract of saffron stigmas. The results indicated that its purity was extremely higher, about 13 times, more than Fluka product. In spite of our expectation, the Fluka product was not a pure · -crocin sample; five other types of crocins in addition to an unknown impurity were seen in its chromatogram. The purity of crystallized total crocin in this work was more than 97. © 2010 Asian Network for Scientific Information.

Item Type: Article
Additional Information: Cited By :71 Export Date: 16 February 2020 Correspondence Address: Mohajeri, S. A.; Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Uncontrolled Keywords: Crocin Crystallization Extraction Purity Saffron alcohol carotenoid plant extract water article chemistry Crocus high performance liquid chromatography isolation and purification metabolism methodology powder spectrophotometry time ultraviolet spectrophotometry X ray crystallography Carotenoids Chromatography, High Pressure Liquid Crystallography, X-Ray Ethanol Plant Extracts Powders Spectrophotometry, Ultraviolet Time Factors
Subjects: QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib4 lib4
Date Deposited: 02 Mar 2020 08:56
Last Modified: 02 Mar 2020 08:56
URI: http://eprints.mums.ac.ir/id/eprint/13016

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