Immunohistochemistry study of p53 and c-erbb-2 expression in trophoblastic tissue and their predictive values in diagnosing malignant progression of simple molar pregnancy

Hasanzadeh, M. and Sharifi, N. and Farazestanian, M. and Nazemian, S. S. and Sani, F. M. (2016) Immunohistochemistry study of p53 and c-erbb-2 expression in trophoblastic tissue and their predictive values in diagnosing malignant progression of simple molar pregnancy. International Journal of Cancer Management, 9 (3). pp. 1-7.

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Abstract

Background: Finding a tumor marker to predict the aggressive behavior of molar pregnancy in early stages has yet been a topic for studies. Objectives: In this survey we planned to study patients with molar pregnancy to 1) assess the p53 and c-erbB-2 expression in trophoblastic tissue, 2) to study the relationship between their expression intensity and progression of a molar pregnancy to gestational trophoblastic neoplasia, and 3) to determine a cut off value for the amount of p53 and c-erbB-2 expression which might correlate with aggressive behavior of molar pregnancy. Patients and Methods: In a prospective cross sectional study by using a high accuracy technique EnVision™ system for immunohistochemistry staining of molar pregnancy samples, we evaluated p53 and c-erbB-2 expression in cytotrophoblast and syncytiotrophoblast and the correlation of their expression with progression of molar pregnancy to gestational trophoblastic neoplasia (GTN). Normal prostatic tissue and Breast cancer tissue were used as positive controls. Results: We studied 28 patients with simple molar pregnancy (SMP) and 30 with GTN. Cytotrophobalst had significantly higher expression of p53 and c-erbB-2 and syncytiotrophoblast had greater expression of p53 in GTN group as compared to SMP group. The cut off values for percentage of p53 positive immunostained cytotrophoblast and syncytiotrophoblast were 5.5 and 2.5. In c-erbB-2 positive membranous stained cytotrophoblast the cut off was 12.5. Conclusions: Our data suggests that over expression of p53 and c-erbB-2 is associated with malignant progression of molar pregnancy. We encountered that high expression of p53 and c-erbB-2 in trophoblastic cells could predict gestational trophoblastic neoplasia during the early stages. © 2016, Iranian Journal of Cancer Prevention.

Item Type: Article
Additional Information: Cited By :2 Export Date: 16 February 2020 Correspondence Address: Sani, F.M.; Qaem Hospital, Mashhad University of Medical Sciences, Number 36, 9th Malek Ashtar St., Ershad Blvd., Iran; email: fmadanisani@yahoo.com
Uncontrolled Keywords: C-erbB-2 Complete Hydatiform Mole P53 Prognosis epidermal growth factor receptor 2 protein p53 Article clinical article controlled study cross-sectional study cytotrophoblast female human human tissue hydatidiform mole immunohistochemistry malignant transformation predictive value prospective study protein expression syncytiotrophoblast trophoblast trophoblastic tumor
Subjects: WP Gynecology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib4 lib4
Date Deposited: 02 Mar 2020 06:22
Last Modified: 02 Mar 2020 06:22
URI: http://eprints.mums.ac.ir/id/eprint/13022

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