Protective effects of peel and seed extracts of Citrus aurantium on glutamate-induced cytotoxicity in PC12 cell line

Hosseini, A. and Sadeghnia, H. R. and Rajabian, A. (2016) Protective effects of peel and seed extracts of Citrus aurantium on glutamate-induced cytotoxicity in PC12 cell line. Folia Neuropathologica, 54 (3). pp. 265-272.

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Abstract

Oxidative stress and apoptosis contribute to neuronal degeneration in many neurodegenerative diseases such as Alzheimer's disease. Glutamate is a major excitatory neurotransmitter in the central nervous system (CNS) and is considered responsible for the pathogenesis of many neurological disorders. Reactive oxygen species (ROS) production is thought to be involved in glutamate-induced apoptosis process. In this study, the neuroprotective effects of Citrus aurantium in the glutamate-induced rat's adrenal pheochromocytoma cell line (PC12 cells) were investigated. The cell viability and apoptotic cell death were measured using MTT and propidium iodine (PI)-staining methods, respectively. In addition, intracellular ROS and malondialdehyde (MDA) levels were determined by fluorometric methods. The results showed that glutamate cytotoxicity in PC12 cells was accompanied by an increment of MDA content, ROS generation, and apoptotic induction. However, pretreatment with peel and seed extracts of C. aurantium significantly reduced MDA content, ROS generation, and apoptotic cells. All these findings indicated that C. aurantium protected PC12 cells against glutamate-induced apoptosis by inhibiting ROS production. Therefore, the present study supports that C. aurantium extracts possess neuroprotective effects against glutamate-induced toxicity in PC12 cell line. The protective effect of C. aurantium might be attributed to its antioxidant properties.

Item Type: Article
Additional Information: Cited By :4 Export Date: 16 February 2020 CODEN: FONEE
Uncontrolled Keywords: Apoptosis Citrus aurantium Glutamate cytotoxicity Neuroprotection PC12 glutamic acid propidium iodide reactive oxygen metabolite sour orange extract antioxidant neuroprotective agent plant extract antioxidant activity Article cell viability controlled study cytotoxicity limit of quantitation lipid peroxidation MTT assay PC12 cell line plant seed post hoc analysis anatomy and histology animal cell survival chemistry Citrus classification drug effects metabolism oxidative stress rat Animals Antioxidants Neuroprotective Agents PC12 Cells Plant Extracts Rats Reactive Oxygen Species Seeds
Subjects: QT physiology
QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib4 lib4
Date Deposited: 02 Mar 2020 05:47
Last Modified: 02 Mar 2020 05:47
URI: http://eprints.mums.ac.ir/id/eprint/13042

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