The effects of crocin on 6-OHDA-induced oxidative/nitrosative damage and motor behaviour in hemiparkinsonian rats

Hosseini, M. and Rajaei, Z. and Alaei, H. and Tajadini, M. (2016) The effects of crocin on 6-OHDA-induced oxidative/nitrosative damage and motor behaviour in hemiparkinsonian rats. Malaysian Journal of Medical Sciences, 23 (6). pp. 35-43.

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Abstract

Background: Crocin is considered to prevent oxidative stress-related diseases, such as ischemia and Alzheimer’s. The aim of the present investigation was to evaluate the effects of crocin on motor behaviour and 6-OHDA-induced oxidative/nitrosative damage to the striatum in an experimental model of Parkinson’s disease. Methods: Left medial forebrain bundle was lesioned by microinjection of 6-OHDA (16μg in 0.2 ascorbate-saline). Crocin (30 and 60 mg/kg) was injected intraperitoneally three days before surgery until six weeks. Rotational behaviour and biochemical analysis were used to evaluate the effect of crocin in a unilateral 6-OHDA-induced model of Parkinson's disease. Results: The contralateral rotations induced by apomorphine in 6-OHDA lesioned group were highly significant (P < 0.001) as compared to the sham group. Moreover, chronic administration of crocin at doses of 30 and 60 mg/kg over six weeks did not change the rotations. The TBARS and nitrite levels in the striatum were also significantly (P < 0.05) increased in lesioned group. Treatment with crocin at a dose of 60 mg/kg significantly decreased the nitrite levels (P < 0.05) in the striatum. Conclusion: Crocin at a dose of 60 mg/kg could be effective in preventing the nitrosative damage in the striatum. Further investigations using higher doses of crocin is suggested to get the full neuroprotective effects of crocin in Parkinson’s disease. © Penerbit Universiti Sains Malaysia, 2016.

Item Type: Article
Additional Information: Cited By :8 Export Date: 16 February 2020 CODEN: MJMSA Correspondence Address: Rajaei, Z.; Department of Physiology, School of Medicine, Isfahan University of Medical SciencesIran; email: rajaeiz@med.mui.ac.ir
Uncontrolled Keywords: 6-hydroxydopamine Crocin Motor activity Nitric oxide Oxidative stress Parkinson disease apomorphine nitrite oxidopamine thiobarbituric acid reactive substance animal experiment animal model Article biochemical analysis chronic drug administration circling behavior controlled study corpus striatum drug dose comparison experimental parkinsonism forebrain locomotion male nitrosative stress nonhuman rat
Subjects: WE Musculoskeletal system
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib4 lib4
Date Deposited: 02 Mar 2020 05:57
Last Modified: 02 Mar 2020 05:57
URI: http://eprints.mums.ac.ir/id/eprint/13045

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