Evaluate the relationship between class 1 integrons and drug resistance genes in clinical isolates of Pseudomonas aeruginosa

Hosseini, S. M. J. and Niloofar, S. N. and Khaledi, A. and Daymad, S. F. and Esmaeili, D. (2016) Evaluate the relationship between class 1 integrons and drug resistance genes in clinical isolates of Pseudomonas aeruginosa. Open Microbiology Journal, 10. pp. 188-196.

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Abstract

Background: The prevalence of resistant Pseudomonas aeruginosa isolates is increasing and it is considered as one of the major public health concerns in the world. The association between integrons and drug resistance has been proven and evidences suggest that integrons are coding and responsible for dissemination of antibiotic resistance among P. aeruginosa isolates. Objective: This study is aimed to evaluate the relationship between class 1 integrons and drug resistance genes in clinical isolates of P. aeruginosa from burn patients. Methods: 100 isolates of P. aeruginosa were collected from burn patients hospitalized in the skin ward of Shahid Motahari hospital and susceptibility testing was performed by disk diffusion method (Kirby-Bauer). Then DNA was extracted and PCR technique was performed for the detection of class 1 integrons and drug resistance genes. Then data was analyzed using SPSS software. Results: The most effective antibiotic was polymyxin B with sensitivity 100, and the most resistance was observed to the ciprofloxacin (93) and amikacin (67), respectively. The maximum and lowest frequencies of drug resistance genes belonged to the aac (6′) - 1, VEB-1 with prevalence rate 93 and 10, respectively. The statistical Chi-square test did not find any significant correlation between class 1 integrons and drug resistance genes (p˃ 0.05). Conclusion: Although no significant correlation between class 1 integrons and drug resistance was observed, but the resistance rate to antibiotics tested among P. aeruginosa isolates was high. So, surveillance, optimization and strict consideration of antimicrobial use and control of infection are necessary. © Hosseini et al.

Item Type: Article
Additional Information: Cited By :10 Export Date: 16 February 2020 Correspondence Address: Esmaeili, D.; Applied Microbiology Research Center, Microbiology Department, Baqiyatallah University of Medical SciencesIran; email: esm114@gmail.com
Uncontrolled Keywords: Burn ward Integrons Pseudomonas aeruginosa Resistance amikacin ceftazidime ciprofloxacin gentamicin polymyxin B tobramycin antibiotic resistance antibiotic sensitivity Article biochemical analysis bioinformatics controlled study correlation analysis disk diffusion DNA extraction frequency analysis gel electrophoresis integron integron 1 nonhuman performance polymerase chain reaction prevalence priority journal Pseudomonas infection
Subjects: QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib4 lib4
Date Deposited: 02 Mar 2020 06:01
Last Modified: 02 Mar 2020 06:01
URI: http://eprints.mums.ac.ir/id/eprint/13046

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