Crocin suppresses multidrug resistance in MRP overexpressing ovarian cancer cell line

Mahdizadeh, S. and Karimi, G. and Behravan, J. and Arabzadeh, S. and Lage, H. and Kalalinia, F. (2016) Crocin suppresses multidrug resistance in MRP overexpressing ovarian cancer cell line. DARU, Journal of Pharmaceutical Sciences, 24 (1).

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Background: Crocin, one of the main constituents of saffron extract, has numerous biological effects such as anti-cancer effects. Multidrug resistance-associated proteins 1 and 2 (MRP1 and MRP2) are important elements in the failure of cancer chemotherapy. In this study we aimed to evaluate the effects of crocin on MRP1 and MRP2 expression and function in human ovarian cancer cell line A2780 and its cisplatin-resistant derivative A2780/RCIS cells. Methods: The cytotoxicity of crocin was assessed by the MTT assay. The effects of crocin on the MRP1 and MRP2 mRNA expression and function were assessed by real-time RT-PCR and MTT assays, respectively. Results: Our study indicated that crocin reduced cell proliferation in a dose-dependent manner in which the reduction in proliferation rate was more noticeable in the A2780 cell line compared to A2780/RCIS. Crocin reduced MRP1 and MRP2 gene expression at the mRNA level in A2780/RCIS cells. It increased doxorubicin cytotoxicity on the resistant A2780/RCIS cells in comparison with the drug-sensitive A2780 cells. Conclusion: Totally, these results indicated that crocin could suppress drug resistance via down regulation of MRP transporters in the human ovarian cancer resistant cell line. © 2016 The Author(s).

Item Type: Article
Additional Information: Cited By :17 Export Date: 16 February 2020 CODEN: DJTSF Correspondence Address: Kalalinia, F.; Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, P. O. Box 91775-1365, Iran; email:
Uncontrolled Keywords: A2780 A2780/RCIS Crocin MRP1 MRP2 Multidrug resistance doxorubicin messenger RNA multidrug resistance associated protein 1 multidrug resistance associated protein 2 antineoplastic agent carotenoid multidrug resistance protein multidrug resistance-associated protein 1 multidrug resistance-associated protein 2 Article cancer cell cancer chemotherapy cell proliferation cytotoxicity down regulation female human human cell MTT assay ovary cancer protein expression protein function real time polymerase chain reaction reverse transcription polymerase chain reaction drug effects drug resistance genetics metabolism Ovarian Neoplasms tumor cell line Antineoplastic Agents Carotenoids Cell Line, Tumor Drug Resistance, Multiple Drug Resistance, Neoplasm Humans Multidrug Resistance-Associated Proteins RNA, Messenger
Subjects: QV pharmacology
QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib4 lib4
Date Deposited: 01 Mar 2020 10:03
Last Modified: 01 Mar 2020 10:03

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