Bioactivity of a novel glycolipid produced by a halophilic Buttiauxella sp. and improving submerged fermentation using a response surface method

Marzban, A. and Ebrahimipour, G. and Danesh, A. (2016) Bioactivity of a novel glycolipid produced by a halophilic Buttiauxella sp. and improving submerged fermentation using a response surface method. Molecules, 21 (10).

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Abstract

An antimicrobial glycolipid biosurfactant (GBS), extracted and identified from a marine bacterium, was studied to inhibit pathogenic microorganisms. Production of the GBS was optimized using a statistical method, a response surface method (RSM) with a central composite design (CCD) for obtaining maximum yields on a cost-effective substrate, molasses. The GBS-producing bacterium was identified as Buttiauxella Species in terms of biochemical and molecular characteristics. This compound showed a desirable antimicrobial activity against some pathogens such as E. coli, Bacillus subtilis, Bacillus cereus, Candida albicans, Aspergilus niger, Salmonella enterica. The rheological studies described the stability of the GBS at high values in a range of pH (7-8), temperature (20-60) and salinity (0-3). The statistical optimization of GBS fermentation was found to be pH 7, temperature 33°C, Peptone 1, NaCl 1 and molasses 1. The potency of the GBS as an effective antimicrobial agent provides evidence for its use against food and human pathogens. Moreover, favorable production of the GBS in the presence of molasses as a cheap substrate and the feasibility of pilot scale fermentation using an RSM method could expand its uses in food, pharmaceutical products and oil industries. © 2016 by the authors; licensee MDPI.

Item Type: Article
Additional Information: Cited By :7 Export Date: 16 February 2020 CODEN: MOLEF Correspondence Address: Danesh, A.; Biotechnology Research Center, Mashhad University of Medical SciencesIran; email: DaneshA@mums.ac.ir
Uncontrolled Keywords: Antimicrobial activity Buttiauxella Glycolipid biosurfactant Response surface method antiinfective agent glycolipid surfactant antibiosis biosynthesis chemistry Enterobacteriaceae fermentation infrared spectroscopy mass fragmentography metabolism nuclear magnetic resonance spectroscopy pH salinity temperature Anti-Infective Agents Gas Chromatography-Mass Spectrometry Glycolipids Hydrogen-Ion Concentration Magnetic Resonance Spectroscopy Spectroscopy, Fourier Transform Infrared Surface-Active Agents
Subjects: QU Biochemistry
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib4 lib4
Date Deposited: 01 Mar 2020 10:24
Last Modified: 01 Mar 2020 10:24
URI: http://eprints.mums.ac.ir/id/eprint/13080

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