Protective effects of saffron extract and crocin supplementation on fatty liver tissue of high-fat diet-induced obese rats

Mashmoul, M. and Azlan, A. and Mohtarrudin, N. and Mohd Yusof, B. N. and Khaza'ai, H. and Khoo, H. E. and Farzadnia, M. and Boroushaki, M. T. (2016) Protective effects of saffron extract and crocin supplementation on fatty liver tissue of high-fat diet-induced obese rats. BMC Complementary and Alternative Medicine, 16 (1).

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Abstract

Background: Saffron is the dried stigma of Crocus sativus L. flower which commonly used as a natural remedy to enhance health and even fights disease in the Middle-East and Southeast Asian countries. Methods: This study was aimed to investigate protective effect of saffron extract and crocin in fatty liver tissue of high-fat diet induced obese rats. A total of 36 healthy male Sprague Dawley rats were divided into six groups. Two groups served as controls, a normal diet (ND) and a high-fat diet (HFD). The other four groups were each supplemented with saffron extract and crocin at concentrations of 40 and 80 mg/kg body weight/day for 8 weeks. All groups except ND were fed with HFD until end of the study. At baseline, blood sample was collected for determination of levels of hepatic marker enzymes, including aspartate aminotransferase, alanine aminotransferase, alkaline phosphatise and albumin. Liver sample was collected, weighed and stained with haematoxylin and eosin for further histopathological examination. Results: Saffron extract and crocin at concentrations of 40 and 80 mg/kg had dose-dependently alleviated levels of liver enzymes and histopathological changes in diet-induced obese rat model compared to control (HFD group). Conclusion: This study suggested that saffron extract and crocin supplements have hepatoprotective effect against non-alcoholic fatty liver disease and HFD-induced liver damage. © 2016 The Author(s).

Item Type: Article
Additional Information: Cited By :15 Export Date: 16 February 2020 CODEN: BCAMC Correspondence Address: Azlan, A.; Universiti Putra Malaysia, Department of Nutrition and Dietetics, Faculty of Medicine and Health SciencesMalaysia; email: azrinaaz@upm.edu.my
Uncontrolled Keywords: Crocin Fatty liver High-fat diet Histopathology NAFLD Obesity Saffron extract alanine aminotransferase albumin alkaline phosphatase aspartate aminotransferase Crocus sativus extract eosin hematoxylin liver protective agent unclassified drug plant extract protective agent alanine aminotransferase blood level albumin blood level alkaline phosphatase blood level animal experiment animal model animal tissue Article aspartate aminotransferase blood level controlled study diet induced obesity limit of quantitation lipid diet liver protection male nonalcoholic fatty liver nonhuman rat adipose tissue animal chemistry Crocus disease model drug effects eating liver metabolism organ size Sprague Dawley rat Animals Diet, High-Fat Disease Models, Animal Non-alcoholic Fatty Liver Disease Plant Extracts Protective Agents Rats Rats, Sprague-Dawley
Subjects: WI Digestive System
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib4 lib4
Date Deposited: 01 Mar 2020 10:58
Last Modified: 01 Mar 2020 10:58
URI: http://eprints.mums.ac.ir/id/eprint/13082

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