Evaluation of the cytotoxic effects of Cyperus longus extract, fractions and its essential oil on the PC3 and MCF7 cancer cell lines

Memariani, T. and Hosseini, T. and Kamali, H. and Mohammadi, A. and Ghorbani, M. and Shakeri, A. and Spandidos, D. A. and Tsatsakis, A. M. and Shahsavand, S. (2016) Evaluation of the cytotoxic effects of Cyperus longus extract, fractions and its essential oil on the PC3 and MCF7 cancer cell lines. Oncology Letters, 11 (2). pp. 1353-1360.

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Abstract

Cyperus longus is one of the Iranian endemic species. However, to date, and to the best of our knowledge, there are no availale academic reports on the cytotoxicity of this plant. Thus, this study was carried out to examine the in vitro anti-proliferative and anti-apoptotic effects of Cyperus longus extract, fractions and essential oil (EO) on MCF7 and PC3 cell lines. The chemical constituents of EO were identified using gas chromatography (GC)-mass spectrometry (MS) analysis. The cells were cultured in RPMI-1640 medium and incubated with various concentrations of the plant extract and fractions. Cell viability was quantified by MTT assay following 24, 48 and 72 h of exposure to (12.5-200 μg/ml) of the methanol extract, the dichloromethane (CH2Cl2), ethyl acetate (EtOAc) and water fractions, as well as the EO of the plant. The percentage of apoptotic cells was determined using propidium iodide staining of DNA fragments by flow cytometry (sub-G1 peak). The most effective fraction in the MCF7 cell line was the CH2Cl2 fraction (IC50 after 48 h, 25.34±2.01). The EtOAc fraction (IC50 after 48 h, 35.2±2.69) and the methanol extract (IC50 after 48 h, 64.64±1.64) were also found to be effective. The IC50 values obtained for the PC3 cell line were 37.97±3.87, 51.57±3.87 and 70.33±2.36 for the CH2Cl2 fraction, the EtOAc fraction and the methanol extract, respectively. Based on these data and due to the partial polarity of the most effective fraction (the CH2Cl2 fraction), we also examined the cytotoxicity of the plant EO. The IC50 values after 48 h were 22.25±4.25 and 12.55±3.65 in the PC3 and MCF7 cell lines, respectively. DNA fragmentation assay also confirmed these data. Performing GC-MS analysis for the plant EO revealed that β-himachalene (10.81), α-caryophyllene oxide (7.6), irisone (4.78), β-caryophyllene oxide (4.36), humulene oxide (12), viridiflorol (4.73), aristolone (6.39) and longiverbenone (6.04) were the main constituents. Our results demonstrated that two of the constituents of Cyperus longus, viridiflorol and longiverbenone, should be investigated further as possible promising chemotherapeutic agents in cancer treatment. © 2016, Spandidos Publications. All rights reserved.

Item Type: Article
Additional Information: Cited By :9 Export Date: 16 February 2020 Correspondence Address: Shahsavand, S.; Department of Physiology and Pharmacology, School of Medicine, North Khorasan University of Medical Sciences, P.O. Box 94176-94735, Iran; email: shabnamshahsavand@gmail.com
Uncontrolled Keywords: Apoptosis Cancer Cyperus longus Cytotoxicity Longiverbenone MTT Viridiflorol aristolone beta himachalene caryophyllene oxide Cyperus longus extract essential oil humulene irisone paclitaxel plant extract plant medicinal product unclassified drug animal cell antineoplastic activity Article cell viability controlled study DNA fragmentation assay extraction flow cytometry human human cell IC50 mass fragmentography MCF 7 cell line medicinal plant mouse MTT assay nonhuman
Subjects: QU Biochemistry
QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib4 lib4
Date Deposited: 01 Mar 2020 09:28
Last Modified: 01 Mar 2020 09:28
URI: http://eprints.mums.ac.ir/id/eprint/13088

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