Brush border membrane vesicle and Caco-2 cell line: Two experimental models for evaluation of absorption enhancing effects of saponins, bile salts, and some synthetic surfactants

Moghimipour, E. and Tabassi, S. A. S. and Ramezani, M. and Handali, S. and Lobenberg, R. (2016) Brush border membrane vesicle and Caco-2 cell line: Two experimental models for evaluation of absorption enhancing effects of saponins, bile salts, and some synthetic surfactants. Journal of Advanced Pharmaceutical Technology and Research, 7 (3). pp. 75-79.

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Abstract

The aim of this study was to investigate the influence of absorption enhancers in the uptake of hydrophilic compounds. The permeation of the two hydrophilic drug models gentamicin and 5 (6)-carboxyfluorescein (CF) across the brush border membrane vesicles and Caco-2 cell lines were evaluated using total saponins of Acanthophyllum squarrosum, Quillaja saponaria, sodium lauryl sulfate, sodium glycocholate, sodium taurodeoxycholate , and Tween 20 as absorption enhancers. Transepithelial electrical resistance (TEER) measurement was utilized to assess the paracellular permeability of cell lines. Confocal laser scanning microscopy (CLSM) was performed to obtain images of the distribution of CF in Caco-2 cells. These compounds were able to loosen tight junctions, thus increasing paracellular permeability. CLSM confirmed the effect of these absorption enhancers on CF transport across Caco-2 lines and increased the Caco-2 permeability via transcellular route. It was also confirmed that the decrease in TEER was transient and reversible after removal of permeation enhancers. © 2016 Journal of Advanced Pharmaceutical Technology & Research | Published by Wolters Kluwer - Medknow.

Item Type: Article
Additional Information: Cited By :5 Export Date: 16 February 2020 Correspondence Address: Handali, S.; Nanotechnology Research Center, Ahvaz Jundishapur University of Medical SciencesIran; email: handalis81@yahoo.com
Uncontrolled Keywords: Absorption enhancers Brush border membrane vesicles Caco-2 cell Transepithelial electrical resistance 6 carboxyfluorescein Acanthophyllum squarrosum saponin bile salt dodecyl sulfate sodium gentamicin glycocholate sodium polysorbate 20 Quillaja saponin saponin surfactant synthetic surfactant taurodeoxycholic acid unclassified drug Article biological model brush border membrane vesicle CACO 2 cell line cell membrane permeability cellular distribution concentration (parameters) confocal laser microscopy controlled study critical micelle concentration electric resistance experimental design hydrogen bond intestine absorption ionic strength lipid bilayer membrane permeability molecular dynamics molecular interaction paracellular permeability priority journal steady state tight junction water content
Subjects: QT physiology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib4 lib4
Date Deposited: 01 Mar 2020 07:55
Last Modified: 01 Mar 2020 07:55
URI: http://eprints.mums.ac.ir/id/eprint/13101

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