Detection of nucleoproteins in squamous cell carcinoma, and dysplastic and normal mucosa in the oral cavity by methyl green-pyronin staining

Mohtasham, N. and Mahdavi-Shahri, N. and Salehinejad, J. and Ejtehadi, H. and Torabi-Parizi, M. and Ghazi, N. (2010) Detection of nucleoproteins in squamous cell carcinoma, and dysplastic and normal mucosa in the oral cavity by methyl green-pyronin staining. Journal of oral science, 52 (2). pp. 239-243.

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Abstract

Squamous cell carcinoma (SCC) is the most malignant neoplasm of the oral cavity. The mortality rate associated with head and neck cancers remains high, and thus detection techniques are important. In the present study, methyl green-pyronin staining was applied to 5 normal mucosa specimens, 10 cases of dysplastic mucosa, 12 cases of SCC (grade I), 10 cases of SCC (grade II) and 5 cases of SCC (grade III) in the oral cavity. This is the first study in which nuclear and nucleolar cytomorphometric parameters (diameter, area and number) in such oral lesions were evaluated using this method. As methyl green-pyronin selectively stains nucleic acids, it can differentiate between DNA (green) and RNA (red). The results reveal that the proposed method can be used to evaluate changes in the nucleus and nucleolus in premalignant lesions in the oral cavity.

Item Type: Article
Additional Information: Cited By :12 Export Date: 16 February 2020 Correspondence Address: Mohtasham, N.
Uncontrolled Keywords: coloring agent diagnostic agent DNA methyl green nucleoprotein pyronine RNA article cell nucleus differential diagnosis epithelium cell histology human methodology mouth mucosa mouth tumor nucleolus pathology photography precancer squamous cell carcinoma ultrastructure Carcinoma, Squamous Cell Cell Nucleolus Coloring Agents Diagnosis, Differential Epithelial Cells Histocytological Preparation Techniques Humans Mouth Neoplasms Nucleoproteins Precancerous Conditions
Subjects: WU Dentistry. Oral surgery
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib4 lib4
Date Deposited: 01 Mar 2020 08:16
Last Modified: 01 Mar 2020 08:16
URI: http://eprints.mums.ac.ir/id/eprint/13106

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