Effects of pomegranate seed oil on oxidative stress markers, serum biochemical parameters and pathological findings in kidney and heart of streptozotocin-induced diabetic rats

Mollazadeh, H. and Sadeghnia, H. R. and Hoseini, A. and Farzadnia, M. and Boroushaki, M. T. (2016) Effects of pomegranate seed oil on oxidative stress markers, serum biochemical parameters and pathological findings in kidney and heart of streptozotocin-induced diabetic rats. Renal Failure, 38 (8). pp. 1256-1266.

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Abstract

Purpose: Oxidative stress due to hyperglycemia is a major cause of diabetes complications. The aim of this study was to evaluate the effects of pomegranate seed oil (PSO) on serum biochemical parameters, cardiomyopathy and nephropathy induced by diabetes mellitus. Method: W/A adult rats were divided into four groups (12 each): group 1, received saline (1 mL/kg), group 2, received streptozotocin (STZ, 65 mg/kg, a single dose as i.p.), groups 3 and 4, received STZ + PSO (0.4 and 0.8 mL/kg, daily by gavage, respectively). After three weeks, six rats of each group and one week later the remaining animals were anesthetized, blood samples were taken for measuring serum biochemical parameters. Sections of heart and kidneys were used for histopathological studies and the remaining tissues were homogenized for measuring malondialdehyde (MDA) and total sulfhydryl groups. Results: Significant elevation of serum creatinine and urea, LDL, triglyceride, glucose levels as well as urine markers, MDA levels in tissue homogenates and a significant decrease in total thiol content and serum HDL were observed in STZ-treated group as compared with control group. PSO treatment resulted in a significant decrease in tissue MDA content, serum creatinine and urea levels as well as urine markers as compared with STZ-treated group. Lipid profile was ameliorated with PSO treatment. PSO also significantly reversed STZ-induced depletion in thiol content and histological abnormality. Effect of PSO was more specific at 28th than 21th days of study. Conclusion: The results showed that PSO has a protective effect against diabetes complications in rats. © 2016 Informa UK Limited, trading as Taylor & Francis Group.

Item Type: Article
Additional Information: Cited By :12 Export Date: 16 February 2020 CODEN: REFAE Correspondence Address: Boroushaki, M.T.; Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical SciencesIran; email: boroushakimt@mums.ac.ir
Uncontrolled Keywords: Diabetes mellitus nephropathy oxidative stress pomegranate seed oil streptozotocin antioxidant C reactive protein cholesterol creatine kinase creatinine glucose high density lipoprotein cholesterol lactate dehydrogenase low density lipoprotein cholesterol malonaldehyde pomegranate extract thiobarbituric acid reactive substance thiol group triacylglycerol unclassified drug urea vegetable oil biological marker streptozocin adult animal experiment animal model animal tissue Article biochemical analysis blood sampling body weight cholesterol blood level controlled study creatinine blood level diabetic cardiomyopathy diabetic nephropathy drug effect enzyme activity glucose blood level glucose urine level histopathology lipid peroxidation male mortality nonhuman outcome assessment pomegranate priority journal protein urine level rat renal protection streptozotocin-induced diabetes mellitus tissue homogenate triacylglycerol blood level urea blood level urine volume animal blood cardiac muscle Cardiomyopathies chemistry complication drug effects experimental diabetes mellitus kidney Kidney Diseases Lythraceae pathology plant seed Wistar rat Animals Biomarkers Diabetes Mellitus, Experimental L-Lactate Dehydrogenase Malondialdehyde Myocardium Plant Oils Punicaceae Rats Rats, Wistar Seeds
Subjects: QY Clinical Pathology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib4 lib4
Date Deposited: 01 Mar 2020 09:21
Last Modified: 01 Mar 2020 09:21
URI: http://eprints.mums.ac.ir/id/eprint/13110

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