Upregulation of cyclin-dependent kinase 7 and matrix metalloproteinase-14 expression contribute to metastatic properties of gastric cancer

Naseh, G. and Mohammadifard, M. and Mohammadifard, M. (2016) Upregulation of cyclin-dependent kinase 7 and matrix metalloproteinase-14 expression contribute to metastatic properties of gastric cancer. IUBMB Life. pp. 799-805.

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Abstract

The aim of this study was to evaluate the protein and mRNA expressions of matrix metalloproteinase-14 (MMP14) and CDK7 in gastric cancer (GC) tissues. Upregulation of MMP14 mRNA level was observed in GC tissues when compared with the matched normal tissues (mean ± SD: 3.92 ± 1.15 vs. 1.35 ± 0.81, P < 0.001). This study indicated that mRNA levels of CDK7 were statistically overexpressed in GC when compared with matched normal tissues (4.12 ± 0.84 vs. 1.43 ± 0.71, P < 0.001). The protein levels of MMP14 were found to be increased in GC (60.41; P < 0.001). The expression of CDK7 was higher in GC tissues than matched normal tissues (70.83; P < 0.001). We found that high MMP14 expression was related to advanced TNM stage (P = 0.004), tumor grade (P = 0.002), and lymph node metastasis (P = 0.015), but no association with other clinical variables (P > 0.05). In addition, high expression of CDK7 was significantly linked to advanced TNM stage (P = 0.001), pathological grade (P = 0.012), and presence of lymph node metastasis (P = 0.009), while no correlation between CDK7 expression and other clinical variables, such as age and gender, distance metastasis. The patients with high expression of MMP14 and CDK7 exhibited worse survival time than those with higher levels. Cox multivariate regression analysis clearly showed that high expression of MMP14 and CDK7 was independent prognostic factors for overall survival in patients with GC. Taken together, these results indicated the overexpression of above markers in the progression and the tumorigenesis of GC and overall patient survival. © 2016 IUBMB Life, 68(10):799–805, 2016. © 2016 International Union of Biochemistry and Molecular Biology

Item Type: Article
Additional Information: Cited By :10 Export Date: 16 February 2020 CODEN: IULIF Correspondence Address: Mohammadifard, M.; Department of Radiology, Imam Reza Hospital, Birjand University of Medical SciencesIran; email: mahyar.mohammadifard@yahoo.com
Uncontrolled Keywords: expression gastric metastasis MMP14 and CDK7 survival cyclin dependent kinase 7 matrix metalloproteinase 14 messenger RNA adult aged Article cancer grading cancer prognosis cancer staging cancer survival cancer tissue controlled study distant metastasis female gene expression human human tissue lymph node metastasis major clinical study male multivariate logistic regression analysis overall survival protein expression real time polymerase chain reaction retrospective study stomach cancer survival time upregulation Western blotting
Subjects: WI Digestive System
QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib4 lib4
Date Deposited: 01 Mar 2020 05:38
Last Modified: 01 Mar 2020 05:38
URI: http://eprints.mums.ac.ir/id/eprint/13124

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