St. John’s wort and its component hyperforin alleviate experimental autoimmune encephalomyelitis through expansion of regulatory T-cells

Nosratabadi, R. and Rastin, M. and Sankian, M. and Haghmorad, D. and Tabasi, N. and Zamani, S. and Aghaee, A. and Salehipour, Z. and Mahmoudi, M. (2016) St. John’s wort and its component hyperforin alleviate experimental autoimmune encephalomyelitis through expansion of regulatory T-cells. Journal of Immunotoxicology, 13 (3). pp. 364-374.

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Abstract

Abstract: Multiple sclerosis (MS) is a central nervous system disorder mainly characterized by inflammation, demyelination and axonal injury. Anti-inflammatory agents can be used to ameliorate the disease process. Hypericum perforatum L or St. John’s wort is widely used as an anti-depressant and anti-inflammatory remedy in traditional and herbal medicine. Based on St. John’s wort properties, the therapeutic potentials of an H. perforatum extract (HPE) and a single component, hyperforin were evaluated for effectiveness against MOG35–55-induced experimental autoimmune encephalomyelitis (EAE), an animal model for human multiple sclerosis. Female C57BL/6 mice were immunized with specific antigen MOG35–55 and then administered different doses of hyperforin or HPE post-immunization. Clinical symptoms/other relevant parameters were assessed daily. Histological analysis of the spinal cord was performed. T-cell proliferative activity was also evaluated using a BrdU assay. The effect of hyperforin on regulatory T-cells (Treg cells) was assessed using flow cytometry. The results indicate hyperforin and HPE reduced the incidence and severity of EAE, an outcome that closely correlated with an inhibition of pathological features (leukocyte infiltration and demyelination) and antigen-specific T-cell proliferation. The study also showed that hyperforin caused increased Treg cell levels in the spleen. These results indicated that hyperforin and HPE could attenuate EAE autoimmune responses by inhibiting immune cell infiltration and expansion of Treg cell and could eventually be considered as a potential candidate for use in the treatment of MS. © 2015 Informa UK Limited trading as Taylor & Francis Group.

Item Type: Article
Additional Information: Cited By :9 Export Date: 16 February 2020 Correspondence Address: Mahmoudi, M.; Immunology Research Center, BuAli Research Institute, School of Medicine, Mashhad University of Medical Sciences, BuAli Square, Iran; email: mahmoudim@mums.ac.ir
Uncontrolled Keywords: Experimental autoimmune encephalomyelitis (EAE) hyperforin Hypericum perforatum L multiple sclerosis myelin oligodendrocyte glycoprotein (MOG) Hypericum perforatum extract myelin oligodendrocyte glycoprotein myelin oligodendrocyte glycoprotein (35-55) peptide fragment phloroglucinol terpene animal experiment animal model animal tissue Article autoimmunity cell expansion drug efficacy drug synthesis experimental autoimmune encephalomyelitis female flow cytometry high performance liquid chromatography histology immunization mouse nonhuman priority journal regulatory T lymphocyte spinal cord weight reduction analogs and derivatives animal C57BL mouse cell proliferation disease model Encephalomyelitis, Autoimmune, Experimental human Hypericum immunology phytotherapy spleen Animals Disease Models, Animal Humans Mice Mice, Inbred C57BL Myelin-Oligodendrocyte Glycoprotein Peptide Fragments T-Lymphocytes, Regulatory Terpenes
Subjects: WB Practice of Medicine
WR Dermatology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib4 lib4
Date Deposited: 01 Mar 2020 06:11
Last Modified: 01 Mar 2020 06:11
URI: http://eprints.mums.ac.ir/id/eprint/13130

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