Elevated caspase-1 activity and IL-1β expression are associated with the IPAF inflammasome in an experimental model of allergy

Nouri, H. R. and Karkhah, A. and Mohammadzadeh, I. and Sankian, M. (2016) Elevated caspase-1 activity and IL-1β expression are associated with the IPAF inflammasome in an experimental model of allergy. Molecular Medicine Reports, 13 (4). pp. 3356-3362.

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Abstract

Previous studies have indicated that interleukin (IL)-1β has an important role in the development of allergic diseases. Therefore, the present study aimed to investigate the upstream pathway underlying IL-1β production in an experimental model of allergy. BALB/c mice (female, 6-8 weeks old) were sensitized to recombinant (r)Che a 2 by intraperitoneal injection of rChe a 2 adsorbed onto an alum gel suspension on days 0, 7, 14 and 21. In the control group, mice received an injection of 20 mM phosphate-buffered saline absorbed onto alum via the same route. The allergic status of the mice was confirmed serologically by measuring allergen-specifi c immunoglobulin (Ig)E levels. The protein expression levels of IL-1β and the mRNA expression levels of inflammasome compartments were measured by enzyme-linked immunosorbent assay and semi-quantitative reverse transcription polymerase chain reaction, respectively. In addition, caspase-1 activity was determined by fluorometric assay. Sensitized mice exhibited significantly increased levels of specific IgE (P<0.05). IL-1β production and caspase-1 activity were significantly higher in the sensitized mice compared with the control group. In addition, no significant differences were observed between the control and sensitized mice in the expression of genes associated with the inflammasome, including NLR family, pyrin domain containing 3; apoptosis-associated speck-like protein; and NLR family, apoptosis inhibitory protein 5. However, IL-1β converting enzyme protease-activating factor (IPAF) expression was significantly increased in sensitized mice compared with in the control group (P<0.05). These data indicate that caspase-1 activation and IL-1β expression are associated with the IPAF inflammasome. Therefore, based on this association, the IPAF inflammasome may be considered for IL-1β production in the experimental model of allergy.

Item Type: Article
Additional Information: Cited By :2 Export Date: 16 February 2020 Correspondence Address: Sankian, M.; Immunobiochemistry Lab, Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Buali Street, Iran; email: sankianm@mums.ac.ir
Uncontrolled Keywords: Allergy Caspase-1 IL-1β Inflammasome IPAF cryopyrin dipeptidyl carboxypeptidase hypoxanthine phosphoribosyltransferase immunoglobulin E interleukin 1beta interleukin 1beta converting enzyme interleukin 1beta converting enzyme protease activating factor messenger RNA NLR family apoptosis inhibitory protein 5 protein unclassified drug apoptosis regulatory protein calcium binding protein Ipaf protein, mouse Naip5 protein, mouse neuronal apoptosis inhibitory protein Nlrp3 protein, mouse animal experiment animal model animal tissue Article comparative study controlled study cytokine production enzyme activation enzyme activity enzyme linked immunosorbent assay female fluorometry immunoglobulin blood level mouse nonhuman protein expression quantitative analysis reverse transcription polymerase chain reaction sensitization tissue homogenate animal Bagg albino mouse disease model genetics hypersensitivity immunology metabolism pathology real time polymerase chain reaction upregulation Animals Apoptosis Regulatory Proteins Calcium-Binding Proteins Caspase 1 Disease Models, Animal Enzyme-Linked Immunosorbent Assay Inflammasomes Interleukin-1beta Mice Mice, Inbred BALB C Neuronal Apoptosis-Inhibitory Protein NLR Family, Pyrin Domain-Containing 3 Protein Real-Time Polymerase Chain Reaction RNA, Messenger Up-Regulation
Subjects: QW Microbiology and Immunology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib4 lib4
Date Deposited: 03 Mar 2020 11:11
Last Modified: 03 Mar 2020 11:11
URI: http://eprints.mums.ac.ir/id/eprint/13131

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