The effects of inflammatory tooth pain on anxiety in adult male rats

Raoof, M. and Ebrahimnejad, H. and Abbasnejad, M. and Amirkhosravi, L. and Raoof, R. and Mahani, S. E. and Ramazani, M. and Shokouhinejad, N. and Khoshkhounejad, M. (2016) The effects of inflammatory tooth pain on anxiety in adult male rats. Basic and Clinical Neuroscience, 7 (3). pp. 259-268.

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Introduction: This study aimed to examine the effects of induced inflammatory tooth pain on anxiety level in adult male rats. Methods: The mandibular incisors of 56 adult male rats were cut off and prefabricated crowns were fixed on the teeth. Formalin and capsaicin were injected intradentally to induce inflammatory tooth pain. Diazepam treated group received diazepam 30 minutes before intradental injection. The anxiety-related behavior was evaluated with elevated plus maze test. Results: Intradental application of chemical noxious stimuli, capsaicin and formalin, significantly affected nociceptive behaviors (P<0.001). Capsaicin (P<0.001) and formalin (P<0.01) significantly increased the anxiety levels in rats by decrease in the duration of time spent in open arm and increase in the duration of time spent in closed arm. Rats that received capsaicin made fewer open arm entries compared to the control animals (P<0.05). Capsaicin (P<0.001) and formalin (P<0.01) treated rats showed more stretch attend postures compared to the control and sham operated animals. In diazepam-pretreated rats, capsaicin induced algesic effect was prevented (P<0.001). Conclusion: Inflammatory pulpal pain has anxiogenic effect on rats, whereas diazepam premedication showed both anxiolytic and pain reducing effects.

Item Type: Article
Additional Information: Cited By :3 Export Date: 16 February 2020 Correspondence Address: Ebrahimnejad, H.; Department of Oral and Maxillofacial Radiology, Faculty of Dentistry, Mashhad University of Medical SciencesIran; email:
Uncontrolled Keywords: Anxiety Capsaicin Diazepam Elevated plus maze Formalin Odontalgia formaldehyde adult animal experiment animal model Article controlled study elevated plus maze test inflammatory pain male nociception nonhuman rat tooth pain
Subjects: WU Dentistry. Oral surgery
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib4 lib4
Date Deposited: 29 Feb 2020 11:18
Last Modified: 29 Feb 2020 11:18

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