Glucose-6-phosphate dehydrogenase deficiency does not increase the susceptibility of sperm to oxidative stress induced by H2O2

Roshankhah, S. and Rostami-Far, Z. and Shaveisi-Zadeh, F. and Movafagh, A. and Bakhtiari, M. and Shaveisi-Zadeh, J. (2016) Glucose-6-phosphate dehydrogenase deficiency does not increase the susceptibility of sperm to oxidative stress induced by H2O2. Clinical and Experimental Reproductive Medicine, 43 (4). pp. 193-198.

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Abstract

Objective: Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common human enzyme defect. G6PD plays a key role in the pentose phosphate pathway, which is a major source of nicotinamide adenine dinucleotide phosphate (NADPH). NADPH provides the reducing equivalents for oxidation-reduction reductions involved in protecting against the toxicity of reactive oxygen species such as H2O2. We hypothesized that G6PD deficiency may reduce the amount of NADPH in sperms, thereby inhibiting the detoxification of H2O2, which could potentially affect their motility and viability, resulting in an increased susceptibility to infertility. Methods: Semen samples were obtained from four males with G6PD deficiency and eight healthy males as a control. In both groups, motile sperms were isolated from the seminal fluid and incubated with 0, 10, 20, 40, 60, 80, and 120 μM concentrations of H2O2. After 1 hour incubation at 37°C, sperms were evaluated for motility and viability. Results: Incubation of sperms with 10 and 20 μM H2O2 led to very little decrease in motility and viability, but motility decreased notably in both groups in 40, 60, and 80 μM H2O2, and viability decreased in both groups in 40, 60, 80, and 120 μM H2O2. However, no statistically significant differences were found between the G6PD-deficient group and controls. Conclusion: G6PD deficiency does not increase the susceptibility of sperm to oxidative stress induced by H2O2, and the reducing equivalents necessary for protection against H2O2 are most likely produced by other pathways. Therefore, G6PD deficiency cannot be considered as major risk factor for male infertility. © 2016. THE KOREAN SOCIETY FOR REPRODUCTIVE MEDICINE.

Item Type: Article
Additional Information: Cited By :3 Export Date: 16 February 2020 Correspondence Address: Bakhtiari, M.; Department of Anatomical Sciences and Biology, Faculty of Medicine, Kermanshah University of Medical SciencesIran; email: mbakhtiari@kums.ac.ir
Uncontrolled Keywords: Glucose-6-phosphate dehydrogenase deficiency Infertility Oxidative stress Risk factors
Subjects: WJ Urogenital System
QU Biochemistry
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib4 lib4
Date Deposited: 29 Feb 2020 08:07
Last Modified: 29 Feb 2020 08:07
URI: http://eprints.mums.ac.ir/id/eprint/13166

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