Effects of aqueous-ethanolic extract of Nigella sativa seeds (Black cumin) and Vitamin E on cisplatin-induced nephrotoxicity in rat

Shafiee, S. and Ebrahimzadeh, A. and Rajaei, Z. and Mohammadian, N. and Alavinezhad, A. and Havakhah, S. and Hossienian, S. and Shahraki, S. and Rad, A. K. (2016) Effects of aqueous-ethanolic extract of Nigella sativa seeds (Black cumin) and Vitamin E on cisplatin-induced nephrotoxicity in rat. Research Journal of Medicinal Plant, 10 (4). pp. 295-302.

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Abstract

Cisplatin (cis-dichlorodiamineplatinum II) is a potent antineoplastic agent against several types of tumor. Nevertheless, its clinical utility is limited due to its cytotoxic adverse effects that include nephrotoxicity. Several factors including inflammation, oxidative stress, DNA damage and mitochondrial injury are involved in cisplatin-induced cell injury. Nigellasativa and vitamin E possess the anti-inflammatory and antioxidant properties. Accordingly, the present study was designed to investigate the protective effects of hydroalcoholic extract of Nigella sativa seeds and vitamin E on cisplatin-induced nephrotoxicity. Forty eight male Wistar rats were randomly divided into 8 groups and treated daily with Nigellasativa extract (100 or 200 mg kg-1, i.p.) or vitamin E (100 mg kg-1, i.p.) 1 h before single dose cisplatin injection (6 mg kg-1, i.p.) and continued for 5 days. Serum and urinary biochemical parameters were measured on day 0 and 6. Histopathological examination of the kidney and determining the kidney index were performed on day 6. The results showed that serum urea and creatinine concentrations, as well as urine glucose, kidney index and histopathological damage were significantly increased in cisplatin group compared with control group, while Glomerular Filtration Rate (GFR) and urea clearance were significantly reduced. Similar alterations in biochemical factors were observed in groups that received cisplatin together with Nigella sativa extract or vitamin E compared to control group. However, treatment with Nigellasativa extract or vitamin E could improve histopathological damages due to cisplatin. In conclusion, Nigella sativa extract or vitamin E treatment attenuated the histological alterations induced by cisplatin. © 2016 Somayeh Shafiee et al.

Item Type: Article
Additional Information: Cited By :2 Export Date: 16 February 2020 Correspondence Address: Rad, A.K.; Department of Physiology, School of Medicine, Mashhad University of Medical SciencesIran
Uncontrolled Keywords: Acute renal failure Cisplatin Nigella sativa Rat Vitamin E alcohol alpha tocopherol creatinine glucose Nigella sativa extract urea water animal experiment animal model animal tissue Article black cumin blood analysis controlled study creatinine blood level glomerulus filtration rate histopathology male nephrotoxicity nonhuman plant seed renal clearance renal protection single drug dose urea blood level urea clearance urinalysis
Subjects: QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib4 lib4
Date Deposited: 29 Feb 2020 04:46
Last Modified: 29 Feb 2020 04:46
URI: http://eprints.mums.ac.ir/id/eprint/13199

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