Effect of total hydroalcholic extract of Nigella sativa and its n-hexane and ethyl acetate fractions on ACHN and GP-293 cell lines

Shahraki, S. and Khajavirad, A. and Shafei, M. N. and Mahmoudi, M. and Tabasi, N. S. (2016) Effect of total hydroalcholic extract of Nigella sativa and its n-hexane and ethyl acetate fractions on ACHN and GP-293 cell lines. Journal of Traditional and Complementary Medicine, 6 (1). pp. 89-96.

[img] Text
1-s2.0-S2225411014000327-main.pdf

Download (1MB)

Abstract

Medicinal plants are noted for their many advantages including the ability to treat diseases such as cancer. In this study, we examined the antitumor effect of the medicinal plant Nigella sativa on the morphology, survival, and apoptosis of ACHN (human renal adenocarcinoma) and GP-293 (normal renal epithelial) cell lines. From a hydroalcoholic extract of N. sativa, n-hexane and ethyl acetate fractions were extracted. Cells were treated with various concentrations of total hydroalcholic extract and n-hexane and ethyl acetate fractions; cell viability, morphological changes, and apoptosis were then determined. Results were presented as mean ± standard error of the mean (SEM). One-way analysis of variance (ANOVA) was applied for the statistical analysis of the data. The total extract and the fractions in a dose- and time-dependent manner reduced the cell viability in ACHN with no effect on the GP-293 cell line. In addition, the total extract resulted in more morphological changes in the ACHN cells compared to the GP-293 cells. The effect of the total extract in inducing apoptosis after 48 hours in the ACHN cell line was greater than in GP-293. In addition, the effect of the two fractions was lower than the total extract at all used concentrations. Therefore, the effect of total extract and n-hexane and ethyl acetate fractions of N. sativa on cell viability and apoptosis in the ACHN cell line is greater than in the GP-293 cell line. However, the effect of the total extract is higher than either of the two fractions on their own. © 2014 Center for Food and Biomolecules, National Taiwan University.

Item Type: Article
Additional Information: Cited By :9 Export Date: 16 February 2020 Correspondence Address: Khajavirad, A.; Neurogenic Inflammation Research Centre, Department of Physiology, School of Medicine, Mashhad University of Medical SciencesIran; email: KhajaviradA@mums.ac.ir
Uncontrolled Keywords: ACHN cell line apoptosis GP-293 cell line MTT Nigella sativa renal cell carcinoma
Subjects: QU Biochemistry
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib4 lib4
Date Deposited: 29 Feb 2020 05:13
Last Modified: 29 Feb 2020 05:13
URI: http://eprints.mums.ac.ir/id/eprint/13202

Actions (login required)

View Item View Item