Detection of aberrant methylated SEPT9 and NTRK3 genes in sporadic colorectal cancer patients as a potential diagnostic biomarker

Sharif, S. B. and Hashemzadeh, S. and Ardehaie, R. M. and Eftekharsadat, A. and Ghojazadeh, M. and Mehrtash, A. H. and Estiar, M. A. and Teimoori-Toolabi, L. and Sakhinia, E. (2016) Detection of aberrant methylated SEPT9 and NTRK3 genes in sporadic colorectal cancer patients as a potential diagnostic biomarker. Oncology Letters, 12 (6). pp. 5335-5343.

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Abstract

Colorectal cancer (CRC) is one of the most common malignancies, and the third leading cause of cancer mortality worldwide. Timely detection of CRC in patients with earlier stages provides the highest rate of survival. Epigenetic alterations are important in the occurrence and progression of CRC, and represent the primary modifications of cancer cells. Therefore, detection of these alterations in CRC cases are thought to hold great promise as diagnostic biomarkers. It has been shown that the SEPT9 and NTRK3 genes are aberrantly methylated and their detection can be used as biomarkers for early diagnosis of CRC. The present study analyzed promoter methylation status of these genes in CRC patients. Genomic DNA was extracted from 45 CRC and paired adjacent healthy tissues and undergone bisulfite conversion, and the methylation status of NTRK3 and SEPT9 were defined using the MS-HRM assay. Our results showed that there are statistically significant differences in methylation status of NTRK3 and specially SEPT9 between CRC and adjacent normal tissues (P<0.001). High sensitivity and specificity for a specific location in SEPT9 gene promoter as a diagnostic biomarker was observed. SEPT9 promoter hypermethylation may serve as a promising biomarker for the detection of CRC development. However, to validate the biomarker potential of NTRK3 there is a requirement for further investigation. © 2016, Spandidos Publications. All rights reserved.

Item Type: Article
Additional Information: Cited By :13 Export Date: 16 February 2020 Correspondence Address: Sakhinia, E.; Department of Bioechemistry and Clinical Laboratory, Division of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Golgasht Street, Iran; email: esakhinia@yahoo.co.uk
Uncontrolled Keywords: Biomarker Colorectal cancer Methylation NTRK3 SEPT9 adult aged Article clinical article controlled study cross-sectional study diagnostic accuracy DNA methylation female gene high resolution melting analysis human human tissue male NTRK3 gene sensitivity and specificity SEPT9 gene
Subjects: WI Digestive System
QZ pathology-Neoplasms
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib4 lib4
Date Deposited: 26 Feb 2020 09:32
Last Modified: 26 Feb 2020 09:32
URI: http://eprints.mums.ac.ir/id/eprint/13207

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