Inorganic arsenic can be potent granulotoxin in mammalian neutrophils in vitro

Taheri, M. and Mehrzad, J. and Afshari, R. and Saleh-Moghaddam, M. and Gharaied, M. H. M. (2016) Inorganic arsenic can be potent granulotoxin in mammalian neutrophils in vitro. Journal of Immunotoxicology, 13 (5). pp. 686-693.

[img] Text
Inorganic arsenic can be potent granulotoxin in mammalian neutrophils in vitro.pdf

Download (1MB)


An important outcome arising out of occupational/environmental exposure to arsenic (As) is immunotoxicity. To determine the impact of inorganic As on innate immune cells, effects of a low dose of NaAsO2 (i.e. 20 ng As/ml) on select parameters associated with human and bovine neutrophils (PMN) were evaluated in vitro. PMN isolated from the blood of healthy individuals and cows (n¼8/treatment) were pre-incubated with NaAsO2 for 12 h before effects on PMN phagocytosis, transcription of TLR2, TLR4 and CD64 in human PMN - as well as on phagocytosis-dependent/-independent cell chemiluminescence (CL), phagocytosis and killing of Staphylococcus aureus and Escherichia coli, PMN H2O2 production and necrosis and TLR4 transcription in bovine PMN - were assessed. Relative to control (no As) PMN, treatment with As significantly decreased phagocytic capacity and CD64 mRNA, but increased TLR2 and TLR4 mRNA, in human PMN. In bovine PMN, while As also led to increased TLR4 mRNA abundance, it resulted in decreases in phagocytosis-dependent and -independent CL, PMN H2O2 production, PMN phagocytosis and killing of both E. coli and S. aureus by PMN. Considering the broad roles of PMN in immunology, the results of these studies increase our understanding of functional consequences of As exposure in inducing immunotoxicity and increasing susceptibility to (infectious) diseases in mammals. © 2016 Informa UK Limited trading as Taylor & Francis Group.

Item Type: Article
Additional Information: Cited By :4 Export Date: 16 February 2020 Correspondence Address: Mehrzad, J.; Immunology Section, Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of MashhadIran; email:
Uncontrolled Keywords: Chemiluminescence Flow cytometry Inorganic arsenic Necrosis Neutrophils TLR arsenic CD64 antigen messenger RNA toll like receptor 2 toll like receptor 4 animal cell Article bactericidal activity blood sampling blood smear chemoluminescence cow Escherichia coli female human human cell immunocompetent cell immunotoxicity in vitro study innate immunity male mammal cell neutrophil nonhuman outcome assessment phagocytosis priority journal RNA analysis Staphylococcus aureus adult animal bovine immunology mammal metabolism young adult Animals Cattle Humans Immunity, Innate Mammals Neutrophils Toll-Like Receptor 2 Toll-Like Receptor 4
Subjects: WA Public Health
QT physiology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib4 lib4
Date Deposited: 26 Feb 2020 07:40
Last Modified: 26 Feb 2020 07:40

Actions (login required)

View Item View Item