Production, assessment, and impregnation of hyaluronic acid with silver nanoparticles that were produced by Streptococcus pyogenes for tissue engineering applications

Yahyaei, B. and Peyvandi, N. and Akbari, H. and Arabzadeh, S. and Afsharnezhad, S. and Ajoudanifar, H. and Pourali, P. (2016) Production, assessment, and impregnation of hyaluronic acid with silver nanoparticles that were produced by Streptococcus pyogenes for tissue engineering applications. Applied Biological Chemistry, 59 (2). pp. 227-237.

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Abstract

Hyaluronic acid (HA) is a mucopolysaccharide with different applications in the medicinal field. Since HA does not have antibacterial property, in this research a bacterium with both abilities of HA and silver nanoparticles (SNPs) production was used; HA containing SNPs (HA/SNPs) was obtained and the toxic effect of HA/SNPs was achieved using Limulus Amebocyte Lysate (LAL) and 3-(4,5 dimethyl thiazol-2yl)-2,5 diphenyl tetrazolium bromide (MTT) assays. HA and SNPs were produced separately by Streptococcus pyogenes. Then HA was analyzed using fourier transform infrared (FTIR) spectroscopy and transmission electron microscope (TEM), and SNPs were analyzed using TEM and X-ray diffraction analysis (XRD). In order to produce HA/SNPs complex, SNPs were added to the supernatant containing HA and the complex was precipitated using isopropyl alcohol. HA, SNPs, and HA/SNPs were used for the antibacterial assay. Results proved the formation of HA and SNPs in the bacterial culture medium. SNPs sizes were around 5–30 nm and entered in the structure of HA. Antibacterial test showed that unlike the pure HA, SNPs and HA/SNPs had antibacterial activity. LAL and MTT assays results showed that HA/SNPs had low and dose-dependent toxic effects. In future, the use of HA/SNPs scaffold in tissue engineering field is expected. © 2016, The Korean Society for Applied Biological Chemistry.

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