In vitro anti-inflammatory and immunomodulatory properties of umbelliprenin and methyl galbanate

Zamani Taghizadeh Rabe, S. and Iranshahi, M. and Mahmoudi, M. (2016) In vitro anti-inflammatory and immunomodulatory properties of umbelliprenin and methyl galbanate. Journal of Immunotoxicology, 13 (2). pp. 209-216.

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Ferula species (Apiaceae) are considered important medicinal plants. The present in vitro study sought to investigate the immunomodulatory and anti-inflammatory properties of terpenoid coumarins isolated from Ferula szowitsiana on immune cells isolated from naïve mice and to elucidate possible underlying mechanisms of action. With splenocytes, effects of the agents on PHA-induced proliferation and interleukin (IL)-4 and interferon (IFN)-γ release were assessed. With peritoneal macrophages, anti-inflammatory potentials were evaluated in lipopolysaccharide (LPS)/IFNγ-stimulated cells via measures of changes in nitric oxide (NO) and PGE2 secretion. Expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) was also determined via Western blot analysis. The results indicated that umbelliprenin (UMB) and methyl galbanate (MG) reduced remarkably PHA-induced splenocyte proliferation and both preferentially induced TH2 IL-4 and suppressed TH1 IFNγ secretion. Each also significantly suppressed LPS-induced production of NO and PGE2 apparently and also led to reductions in inducible iNOS and COX-expression. To the authors knowledge, the present study is the first to report on anti-inflammatory and immunomodulatory effects of UMB and MG in vitro. The present results suggest that each could potentially be exploited as a natural immunosuppressant against inflammatory and autoimmune diseases once substantial further toxicological analyses have been done to confirm overall safety in vivo. © 2015 Informa UK Limited trading as Taylor & Francis Group.

Item Type: Article
Additional Information: Cited By :12 Export Date: 16 February 2020 Correspondence Address: Mahmoudi, M.; Immunology Research Center, Bu-Ali Research Institute, BuAli Square, Iran; email:
Uncontrolled Keywords: Ferula szowitsiana lymphocyte macrophage methyl galbanate umbelliprenin antiinflammatory agent coumarin derivative cyclooxygenase 2 gamma interferon immunomodulating agent inducible nitric oxide synthase interleukin 4 lipopolysaccharide nitric oxide prostaglandin E2 unclassified drug immunologic factor Ptgs2 protein, mouse umbelliferone derivative animal cell antiinflammatory activity Article cell isolation cell proliferation controlled study cytokine production cytokine release drug isolation drug mechanism Ferula immunocompetent cell immunomodulation in vitro study mouse nonhuman peritoneum macrophage priority journal protein expression spleen cell Th1 cell Th2 cell Western blotting animal Bagg albino mouse chemistry immunology spleen Animals Immunologic Factors Interferon-gamma Macrophages, Peritoneal Mice Mice, Inbred BALB C Th1 Cells Th2 Cells Umbelliferones
Subjects: WQ Obstetrics
QW Microbiology and Immunology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib4 lib4
Date Deposited: 04 Mar 2020 04:57
Last Modified: 04 Mar 2020 04:57

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