Diagnostic investigations of DKK-1 and PDCD5 expression levels as independent prognostic markers of human chondrosarcoma

Zarea, M. and Mohammadian Bajgiran, A. and Sedaghati, F. and Hatami, N. and Taheriazam, A. and Yahaghi, E. and Shakeri, M. (2016) Diagnostic investigations of DKK-1 and PDCD5 expression levels as independent prognostic markers of human chondrosarcoma. IUBMB Life. pp. 597-601.

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Abstract

In this study, we investigated the expression levels of Dickkopf-1 (DKK-1) and programmed cell death 5 (PDCD5) by using quantitative real-time PCR and immunohistochemistry in patients with chondrosarcoma. The DKK-1 mRNA levels were significantly higher in chondrosarcoma when compared with the corresponding nontumor tissues (mean ± SD: 4.23 ± 1.54; 1.54 ± 0.87; P = 0.001). PDCD5 mRNA levels were remarkably deceased in tumor tissues when compared with corresponding nontumor tissues (mean ± SD: 1.94 ± 0.73; 5.42 ± 1.73; P = 0.001). The high and moderate DKK-1 expressions were observed for 60 of chondrosarcoma samples in comparison with 27.5 of corresponding nontumor tissues (P = 0.001). Moreover, low expression of PDCD5 was found in 67.5 of the tumor tissues when compared with the nontumor tissues (32.5; P = 0.002). The results of this study showed that high DKK-1 expression levels were strongly related to MSTS stage (P = 0.011) and the advancement of histological grade (P < 0.001). Furthermore, the PDCD5 expression levels were correlated with histological grade (P < 0.001), MSTS stage (P = 0.016), and distant metastasis (P = 0.001). Kaplan-Meier survival and log-rank survival showed that patients with high DKK-1 levels and low PDCD5 levels were correlated with shorter overall survival (log-rank test P < 0.001). PDCD5 levels, histological grade, and tumor stage were independent predictors of overall survival. In conclusion, DKK-1 and PDCD5 can be independent predictors of overall survival in patients suffering from chondrosarcoma. © 2016 IUBMB Life, 68(7):597–601, 2016. © 2016 International Union of Biochemistry and Molecular Biology

Item Type: Article
Additional Information: Cited By :3 Export Date: 16 February 2020 CODEN: IULIF
Uncontrolled Keywords: chondrosarcoma DKK-1 and PDCD5 immunohistochemistry patients PCR dickkopf 1 protein messenger RNA programmed cell death 5 protein protein tumor marker unclassified drug adult Article cancer grading cancer patient cancer prognosis cancer staging cancer survival cancer tissue clinical article controlled study distant metastasis female histopathology human human tissue Kaplan Meier method male overall survival protein expression quantitative analysis real time polymerase chain reaction
Subjects: QU Biochemistry
QW Microbiology and Immunology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib4 lib4
Date Deposited: 04 Mar 2020 04:54
Last Modified: 04 Mar 2020 04:54
URI: http://eprints.mums.ac.ir/id/eprint/13258

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