Rapid detection of Candida species in bronchoalveolar lavage fluid from patients with pulmonary symptoms

Zarrinfar, H. and Kaboli, S. and Dolatabadi, S. and Mohammadi, R. (2016) Rapid detection of Candida species in bronchoalveolar lavage fluid from patients with pulmonary symptoms. Brazilian Journal of Microbiology, 47 (1). pp. 172-176.

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Abstract

Candida species, especially C. albicans, are commensals on human mucosal surfaces, but are increasingly becoming one of the important invasive pathogens as seen by a rise in its prevalence in immunocompromised patients and in antibiotic consumption. Thus, an accurate identification of Candida species in patients with pulmonary symptoms can provide important information for effective treatment. A total of 75 clinical isolates of Candida species were obtained from the bronchoalveolar lavage fluid of both immunocompromised and immunocompetent patients with pulmonary symptoms. Candida cultures were identified based on nuclear ribosomal Internal Transcribed Spacer (ITS1-ITS2 rDNA) sequence analysis by polymerase chain reaction–restriction fragment length polymorphisms (PCR-RFLP).Molecular identification indicated that the isolates belonged predominantly to C. albicans (52), followed by C. tropicalis (24), C. glabrata (14.7), C. krusei (5.3), C. parapsilosis (1.3), C. kefyr (1.3) and C. guilliermondii (1.3). Given the increasing complexity of disease profiles and their management regimens in diverse patients, rapid and accurate identification of Candida species can lead to timely and appropriate antifungal therapy. © 2015 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.

Item Type: Article
Additional Information: Cited By :13 Export Date: 16 February 2020 Correspondence Address: Mohammadi, R.; Department of Medical Parasitology and Mycology, School of Medicine and Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical SciencesIran; email: dr.rasoulmohammadi@yahoo.com
Uncontrolled Keywords: Candida PCR-RFLP Pulmonary fungal DNA ribosomal spacer DNA bronchoalveolar lavage fluid candidiasis chemistry classification DNA sequence genetics human isolation and purification Lung Diseases, Fungal microbiology polymerase chain reaction restriction fragment length polymorphism time factor DNA, Fungal DNA, Ribosomal Spacer Humans Polymorphism, Restriction Fragment Length Sequence Analysis, DNA Time Factors
Subjects: QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib4 lib4
Date Deposited: 04 Mar 2020 04:51
Last Modified: 04 Mar 2020 04:51
URI: http://eprints.mums.ac.ir/id/eprint/13262

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