Discrimination of Trichophyton tonsurans and Trichophyton equinum by PCR-RFLP and by β-tubulin and Translation Elongation Factor 1-α sequencing

Rezaei-Matehkolaei, A. and Makimura, K. and De Hoog, G. S. and Shidfar, M. R. and Satoh, K. and Najafzadeh, M. J. and Mirhendi, H. (2012) Discrimination of Trichophyton tonsurans and Trichophyton equinum by PCR-RFLP and by β-tubulin and Translation Elongation Factor 1-α sequencing. Medical Mycology, 50 (7). pp. 760-764.

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Trichophyton tonsurans and T. equinum are two closely related sister species of dermatophytes, but differ in their preferred hosts, i.e., humans or horses, respectively. Routine procedures for their identification depend on studies of their phenotypic, physiological and biochemical characteristics, which are laborious and may yield ambiguous results. Molecular methods using rDNA ITS also had been judged to be insufficiently discriminatory. In the present study two genetic markers were sequenced in addition to the ITS region, i.e., partial β-tubulin (BT2) and translation elongation factor 1-α (TEF1). The TEF1 locus revealed a consistent differences in a 13 bp indel and an additional SNP between the two species, along with a single base substitution in BT2 and one ITS1, enabling unambiguous distinction of the two taxa. RFLP targeting the ITS region was evaluated as a potential tool for routine screening of suspected isolates of T. tonsurans and T. equinum. © 2012 ISHAM.

Item Type: Article
Additional Information: Cited By :21 Export Date: 16 February 2020 CODEN: MEMYF Correspondence Address: Mirhendi, H.; Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran 14155-6446, Iran; email: mirhendi@tums.ac.ir
Uncontrolled Keywords: β-tubulin ITS PCR-RFLP Translation elongation factor 1-α Trichophyton equinum Trichophyton tonsurans beta tubulin elongation factor 1alpha article bacterial strain consensus sequence dermatophyte nonhuman nucleic acid base substitution nucleotide sequence polymerase chain reaction restriction fragment length polymorphism single nucleotide polymorphism Trichophyton mentagrophytes Animals Base Sequence DNA, Fungal DNA, Ribosomal Spacer Humans INDEL Mutation Molecular Sequence Data Mycology Peptide Elongation Factor 1 Polymorphism, Restriction Fragment Length Polymorphism, Single Nucleotide Sequence Analysis, DNA Trichophyton Tubulin Arthrodermataceae Equidae
Subjects: QX Parasitology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib5 lib5
Date Deposited: 22 Apr 2020 05:36
Last Modified: 22 Apr 2020 05:36
URI: http://eprints.mums.ac.ir/id/eprint/16569

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