Distribution of hepatitis delta virus genotypes in Mashhad, Northeast Iran

Sadeghian, H. and Varasteh, N. and Esmaeelzadeh, A. and Nomani, H. and Alimardani, M. and Davoodnejad, M. and Meshkat, M. and Ahadi, M. and Sepahi, S. and Rostami, S. and Meshkat, Z. (2015) Distribution of hepatitis delta virus genotypes in Mashhad, Northeast Iran. Jundishapur Journal of Microbiology, 8 (2).

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Abstract

Background: Hepatitis delta virus (HDV) is dependent on the hepatitis B virus for transmission and propagation. Based on isolated HDV sequences from different parts of the world, at least three major different genotypes with different geographic distributions are suggested. Studies have shown that genotype 1 is the predominant genotype of HDV in different parts of Iran; however, the genotype distribution of this virus has not been identified in Mashhad, northeast Iran. Objectives: This current study determines the frequency of HDV major genotypes in Mashhad, Iran. Patients and Methods: Twenty-five participants were enrolled in this study. All samples were positive for HBsAg (determined by Enzyme-linked immunosorbent assay (ELISA)) and anti-HDV. RNA extraction and cDNA synthesis was performed. Then, PCR was performed and HDV genotypes were determined by restriction fragment length polymorphism (RFLP). Results: Of 25 patients, 12 (48) were positive for HDV RNA. Genotype analysis of HDV RNA revealed that the prevalence of HDV genotypes I and II was 83.3 (n = 10) and 16.7 (n = 2), respectively. Conclusions: This study showed that the most prevalent genotype of HDV in Mashhad was genotype I. It was of interest that in contrast to other provinces of Iran, HDV genotype 2 was observed in Mashhad. Similar studies with larger sample sizes could provide valuable information regarding the molecular epidemiology and geographical distribution. It may also help control and prevent the spread of hepatitis D virus infections. In addition, the genotyping of HDV may predict the severity of the disease. © 2015, Ahvaz Jundishapur University of Medical Sciences.

Item Type: Article
Additional Information: Cited By :4 Export Date: 16 February 2020 Correspondence Address: Meshkat, Z.; Department of Human Genetic, School of Medicine, Mashhad University of Medical SciencesIran
Uncontrolled Keywords: Genotype Hepatitis delta virus Polymerase chain reaction Restriction fragment length polymorphism hepatitis B antibody hepatitis delta antigen adult antibody detection Article cladistics clinical article delta agent hepatitis disease severity DNA synthesis enzyme linked immunosorbent assay female gene frequency genetic analysis geographic distribution human Iran male molecular epidemiology nonhuman prediction prevalence RNA extraction Hepatitis B virus
Subjects: WC Communicable Diseases
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib5 lib5
Date Deposited: 04 Apr 2020 05:32
Last Modified: 04 Apr 2020 05:32
URI: http://eprints.mums.ac.ir/id/eprint/16590

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