Protective effect of silymarin against acrolein-induced cardiotoxicity in mice

Taghiabadi, E. and Imenshahidi, M. and Abnous, K. and Mosafa, F. and Sankian, M. and Memar, B. and Karimi, G. (2012) Protective effect of silymarin against acrolein-induced cardiotoxicity in mice. Evidence-based Complementary and Alternative Medicine, 2012.

[img] Text
352091.pdf

Download (5MB)
Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Reactive α,β-unsaturated aldehydes such as acrolein (ACR) are major components of environmental pollutants and have been implicated in the neurodegenerative and cardiac diseases. In this study, the protective effect of silymarin (SN) against cardiotoxicity induced by ACR in mice was evaluated. Studies were performed on seven groups of six animals each, including vehicle-control (normal saline + 0.5 w/v methylcellulose), ACR (7.5 mg/kg/day, gavage) for 3 weeks, SN (25, 50 and 100 mg/kg/day, i.p.) plus ACR, vitamin E (Vit E, 100 IU/kg, i.p.) plus ACR, and SN (100 mg/kg, i.p.) groups. Mice received SN 7 days before ACR and daily thereafter throughout the study. Pretreatment with SN attenuated ACR-induced increased levels of malondialdehyde (MDA), serum cardiac troponin I (cTnI), and creatine kinase-MB (CK-MB), as well as histopathological changes in cardiac tissues. Moreover, SN improved glutathione (GSH) content, superoxide dismutase (SOD), and catalase (CAT) activities in heart of ACR-treated mice. Western blot analysis showed that SN pretreatment inhibited apoptosis provoked by ACR through decreasing Bax/Bcl-2 ratio, cytosolic cytochrome c content, and cleaved caspase-3 level in heart. In conclusion, SN may have protective effects against cardiotoxicity of ACR by reducing lipid peroxidation, renewing the activities of antioxidant enzymes, and preventing apoptosis. © 2012 Elahe Taghiabadi et al.

Item Type: Article
Additional Information: Cited By :22 Export Date: 16 February 2020 Correspondence Address: Karimi, G.; Medical Toxicology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad 9177948564, Iran; email: karimig@mums.ac.ir
Uncontrolled Keywords: acrolein alpha tocopherol caspase 3 catalase creatine kinase MB cytochrome c glutathione malonaldehyde methylcellulose protein Bax protein bcl 2 silymarin superoxide dismutase troponin I animal experiment animal model apoptosis article cardiotoxicity controlled study enzyme activity heart protection histopathology lipid peroxidation male mouse nonhuman oxidative stress priority journal Western blotting
Subjects: WG Cardiovascular System
QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib5 lib5
Date Deposited: 11 Mar 2020 09:00
Last Modified: 11 Mar 2020 09:00
URI: http://eprints.mums.ac.ir/id/eprint/16633

Actions (login required)

View Item View Item