Wogonin and neobaicalein from Scutellaria litwinowii roots are apoptotic for HeLa cells

Tayarani-Najarani, Z. and Asili, J. and Parsaee, H. and Mousavi, S. H. and Mashhadian, N. V. and Mirzaee, A. and Emami, S. A. (2012) Wogonin and neobaicalein from Scutellaria litwinowii roots are apoptotic for HeLa cells. Brazilian Journal of Pharmacognosy, 22 (2). pp. 268-276.

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Abstract

Chemical investigation on the CH 2Cl 2 fraction of the Scutellaria litwinowii Bornm. & Sint., Lamiaceace, root extract for the fi rst time resulted in the isolation of wogonin, and neobaicalein. These compounds were evaluated for their cytotoxicity towards HeLa cell lines and lymphocytes. Meanwhile, the role of apoptosis was explored in this toxicity. The cells were cultured in RPMI medium and incubated with different concentrations of isolated fl avonoids. Cell viability was quantifi ed by MTS assay. Apoptotic cells were determined using propidium iodide staining of DNA fragmentation by fl ow cytometry (sub-G1peak). Wogonin, and neobaicalein inhibited the growth of malignant cells in a dose-dependent manner. The IC50 values of 46.62 and 79.34 μM were, respectively, found for neobaicalein and wogonin against HeLa cells after 48 h of treatment. Neobaicalein induced a sub-G1 peak in the fl ow cytometry histogram of treated cells compared to control cells indicating that apoptotic cell death is involved in neobaicalein toxicity. Neobaicalein exerts cytotoxic and pro-apoptotic effects in HeLa cell lines and could be considered as a potential chemotherapeutic agent in cancer treatment.

Item Type: Article
Additional Information: Cited By :11 Export Date: 16 February 2020 Correspondence Address: Emami, S. A.; Department of Pharmacognosy, School of Pharmacy, Mashhad, University of Medical Science, P.O. Box 9177948564, Mashhad, Iran; email: emamia@mums.ac.ir
Uncontrolled Keywords: Apoptosis Lamiaceae Neobaicalein Scutellaria litwinowii Wogonin 6 hydroxyflavone baicalein DNA plant extract propidium iodide Scutellaria litwinowii extract unclassified drug antineoplastic activity article cancer inhibition cell culture cell death cell viability controlled study cytotoxicity DNA fragmentation dose response drug isolation flow cytometry growth inhibition HeLa cell histogram human human cell incubation temperature lymphocyte medicinal plant nonhuman structure activity relation
Subjects: WB Practice of Medicine
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib5 lib5
Date Deposited: 04 Mar 2020 05:17
Last Modified: 04 Mar 2020 05:17
URI: http://eprints.mums.ac.ir/id/eprint/16673

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