Angiogenic activity of endothelial progenitor cells through angiopoietin-1 and angiopoietin-2

Siavashi, V. and Sariri, R. and Nassiri, S. M. and Esmaeilivand, M. and Asadian, S. and Cheraghi, H. and Barekati-Mowahed, M. and Rahbarghazi, R. (2016) Angiogenic activity of endothelial progenitor cells through angiopoietin-1 and angiopoietin-2. Animal Cells and Systems, 20 (3). pp. 118-129.

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Angiogenesis is a regulated process involving the proliferation, migration, and remodeling of different cell types particularly mature endothelial cells and recently discovered progenitor cells, named as endothelial progenitor cells (EPCs). Up to now, many attempts have been made to understand the dynamic balance of pro- and anti-angiogenic factors on EPCs on different milieu. It has been accepted that Ang-1, -2 and Tie-1, -2 signaling play a key role on angiogenesis pathways in endothelial lineage cells. In the current experiment, the angiogenic/angio-modulatory potency of Ang-1 and -2 was investigated on isolated EPCs. Freshly isolated EPCs were exposed to different concentrations of Ang-1 and -2 (25 and 50 ng/ml) over a course of 7 and 14 days. Corroborating to our results, a superior effect of Ang-1 on angiogenic properties, including an increased concentration of vascular endothelial growth factor, in vitro tubulogenesis, EPC migratory, Tie-2 expression and clonogenicity, was determined. A large amount of positive mature endothelium markers was achieved in EPCs being-exposed to Ang-1 peptide. Nonetheless, the number of CD133 positive cells increased in the presence of Ang-2. Collectively, we conclude that Ang-1 potentially induces functional and mature vascular-like behavior in EPCs more than Ang-2. © 2016 Korean Society for Integrative Biology.

Item Type: Article
Additional Information: Cited By :14 Export Date: 16 February 2020 Correspondence Address: Sariri, R.; Department of Biology, Faculty of Sciences, University of GuilanIran; email:
Uncontrolled Keywords: angiogenic activity Angiopoietin-1 angiopoietin-2 endothelial progenitor cells
Subjects: QT physiology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib4 lib4
Date Deposited: 04 Mar 2020 04:19
Last Modified: 04 Mar 2020 04:19

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