Detection of p53 exon 9 gene mutation in bladder cancer by polymerase chain reaction single strand conformation polymorphism method

Abbasi, M. and Mirmomeni, M. H. and Khazaei, S. and Ranjbaran, R. and Bidmeshkipoor, A. (2017) Detection of p53 exon 9 gene mutation in bladder cancer by polymerase chain reaction single strand conformation polymorphism method. Indian Journal of Medical Sciences, 69 (1). pp. 47-51.

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Abstract

Introduction: Urinary bladder cancer is the fourth most common diagnosed malignancy in men and the tenth in women worldwide. The successful treatment depends on early detection and specific diagnostic approaches. Mutations in the p53 tumor suppressor gene (TP53) are very common among the cancers, especially in bladder cancer that p53 mutation is identified in around 60 percents. Aims: In this study, we aim to detect p53 mutation in bladder cancer patients in Kermanshah city of Iran. Materials and Methods: Thirty paraffin embedded specimens were obtained from bladder cancer patients. They were reviewed by pathologists for substantial amounts of neoplastic tissue. PCR SSCP technique was used followed by standard silver staining to analyze TP53 exon 9 gene mutations in tumor samples. RESULTS: Polyacrylamide gel analysis represented mutations in 2 cases (6.7) that were classified as p53 positive, and there were no mutations in 28 cases (93.3) that were classified as p53 negative. No significant correlation observed between p53 mutation and histological grade, tumor stage, muscular propria status, patient sex, and age. Conclusion: These data are the first report on the p53 abnormalities in bladder cancer patients from Kermanshah. We can suppose that the percentage of p53 exon 9 mutation is low in bladder cancer patients from western Iran. However, further studies on a large number of tissue specimens, if possible, help to verify the obtained results.

Item Type: Article
Additional Information: Cited By :1 Export Date: 16 February 2020 CODEN: INJMA Correspondence Address: Abbasi, M.; Department of Biology, School of Science, Razi UniversityIran; email: mabbasi1982@yahoo.com
Uncontrolled Keywords: Bladder cancer Mutation P53 Polymerase chain reaction Single-strand conformation polymorphism protein p53 adult aged Article cancer staging clinical article colonic muscle controlled study DNA extraction exon female gene mutation histology human human tissue male nested polymerase chain reaction polyacrylamide gel electrophoresis silver staining single strand conformation polymorphism tumor suppressor gene
Subjects: WJ Urogenital System
QU Biochemistry
QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib3 lib3
Date Deposited: 23 May 2020 07:20
Last Modified: 23 May 2020 07:20
URI: http://eprints.mums.ac.ir/id/eprint/16720

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