p16 promoter hypermethylation: A useful serum marker for early detection of gastric cancer

Abbaszadegan, M. R. and Moaven, O. and Sima, H. R. and Ghafarzadegan, K. and A'Rabi, A. and Forghani, M. N. and Raziee, H. R. and Mashhadinejad, A. and Jafarzadeh, M. and Esmaili-Shandiz, E. and Dadkhah, E. (2008) p16 promoter hypermethylation: A useful serum marker for early detection of gastric cancer. World Journal of Gastroenterology, 14 (13). pp. 2055-2060.

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Abstract

Aim: To determine p16 promoter hypermethylation in gastric tumoral tissue and serum samples, its impact on p16-protein expression, and correlation with clinical and histological features. Methods: Samples were obtained from 52 histologically confirmed cases of gastric adenocarcinoma. Gastric tissue and serum of 50 age- and sex-matched individuals with normal gastroscopy and biopsy were obtained as control samples. Methylation-specific polymerase chain reaction (MSP) was used to evaluate methylation status or p16 promoter. p16-protein expression was analyzed by immunohistochemical staining on paraffin-embedded sections. Results: Methylation was detected in 44.2 (23/52) of tumoral tissues. 60.9 of them were also methylated in serum, i.e., 26.9 of all patients (14/52). Methylation was not detected in tissue and sera of control samples. p16-protein expression was decreased in 61.5 of cases (32/52), and was significantly associated with promoter hypermethylation (P < 0.001). Methylation was significantly more frequent in higher pathological grades (P < 0.05). Methylation was not associated with other clinicopathological features and environmental factors including H-pylori infection and smoking. Conclusion: p16 promoter hypermethylation is an important event in gastric carcinogenesis. It is the principle mechanism of p16 gene silencing. It is related to malignant tumor behavior. Detection of DNA methylation in serum may be a biomarker for early detection of gastric cancer. © 2008 WJG. All rights reserved.

Item Type: Article
Additional Information: Cited By :74 Export Date: 16 February 2020 CODEN: WJGAF Correspondence Address: Abbaszadegan, M.R.; Division of Human Genetics, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad 9196773117, Iran; email: abbaszadeganmr@mums.ac.ir
Uncontrolled Keywords: Gastric cancer Hypermethylation Methylation specific PCR p16 biochemical marker protein p16 aged article bacterial infection blood sampling clinical article DNA hypermethylation DNA methylation early diagnosis environmental factor female gastroscopy gene silencing Helicobacter pylori histopathology human human cell immunochemistry male polymerase chain reaction promoter region protein analysis protein expression smoking stomach biopsy stomach cancer stomach carcinogenesis Humans Immunohistochemistry Middle Aged Models, Biological Neoplasm Proteins Promoter Regions (Genetics) Stomach Neoplasms Sulfites Tumor Markers, Biological
Subjects: WI Digestive System
QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib3 lib3
Date Deposited: 13 May 2020 05:22
Last Modified: 13 May 2020 05:22
URI: http://eprints.mums.ac.ir/id/eprint/16721

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