Downregulation of Caspase 8 in a group of Iranian breast cancer patients – A pilot study

Aghababazadeh, M. and Dorraki, N. and Javan, F. A. and Fattahi, A. S. and Gharib, M. and Pasdar, A. (2017) Downregulation of Caspase 8 in a group of Iranian breast cancer patients – A pilot study. Journal of the Egyptian National Cancer Institute, 29 (4). pp. 191-195.

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Abstract

Purpose It is now well known that evading apoptosis, as a cancer hallmark, can lead to tumour initiation, progression and metastasis. As a result of genome wide association studies, an initiator protease in this pathway, caspase 8 (CASP8), has been found to be an important gene regarding breast cancer susceptibility. The alterations of the expression of this gene have been reported in breast cancer cell lines. Given that in previous studies expression analysis of this gene had only been done in breast cancer cell lines, in this study we aimed to evaluate the expression of this gene in breast cancer tissues versus adjacent normal tissues, using real-time quantitative method. Methods Caspase 8 mRNA expression was quantified using comparative RT-qPCR in 27 fresh frozen breast tumours and 27 adjacent normal tissues. Moreover, relationship between the expression changes of CASP8 in tumour tissue and various clinical and pathological features were evaluated in an Iranian population. Results The present study showed that expression of CASP8 was significantly reduced in tumour tissues compared to neighbouring normal tissues (p =.004). CASP8 expression was significantly correlated with the status of hormone receptors (ER and PR). Conclusion To the best of our knowledge, this study is the first report on reduced expression of CASP8 in breast cancer versus adjacent normal tissues. Our data support previous results obtained from cell lines and therefore highlights the seminal role of the induction of CASP8 expression, as a novel therapeutic approach, in order to sensitize tumour cells to apoptotic stimuli. © 2017 National Cancer Institute, Cairo University

Item Type: Article
Additional Information: Cited By :4 Export Date: 16 February 2020 Correspondence Address: Pasdar, A.; Department of Medical Genetics, Faculty of Medicine, Mashhad University of Medical SciencesIran; email: pasdara@mums.ac.ir
Uncontrolled Keywords: Apoptosis Breast cancer Caspase 8 fresh frozen plasma messenger RNA tumor marker adult Article breast cancer cell line breast surgery cancer diagnosis cancer surgery clinical article comparative study controlled study correlational study down regulation female gene expression human human cell human tissue Iranian people middle aged pilot study protein expression real time polymerase chain reaction tumor volume aged breast tumor cancer grading cancer staging gene expression regulation genetics Iran metabolism metastasis pathology tumor cell line Biomarkers, Tumor Breast Neoplasms Cell Line, Tumor Down-Regulation Gene Expression Regulation, Neoplastic Humans Neoplasm Grading Neoplasm Metastasis Neoplasm Staging Pilot Projects Tumor Burden
Subjects: QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib3 lib3
Date Deposited: 13 May 2020 06:40
Last Modified: 13 May 2020 06:40
URI: http://eprints.mums.ac.ir/id/eprint/16727

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