Development of an effective delivery system for intranasal immunization against Mycobacterium tuberculosis ESAT-6 antigen

Amini, Y. and Tebianian, M. and Mosavari, N. and Fasihi Ramandi, M. and Ebrahimi, S. M. and Najminejad, H. and Dabaghian, M. and Abdollahpour, M. (2017) Development of an effective delivery system for intranasal immunization against Mycobacterium tuberculosis ESAT-6 antigen. Artificial Cells, Nanomedicine and Biotechnology, 45 (2). pp. 291-296.

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Abstract

Introduction The early secreted antigenic target 6-kDa protein (ESAT-6) plays an important role in immune protection against Tuberculosis. Owing to its great potential to increase immune response, chitosan can be considered as a suitable biodegradable polymer for intranasal administration. Methods The physiochemical properties of the nanoparticle were measured in vitro. Two weeks after the last intranasal administration, blood samples were collected and specific IgG, IFN-gama, and IL-4 levels were measured by ELISA. Results Chitosan nanoparticles containing ESAT-6 demonstrated stronger ability to induce IFN-gama, IL-4, and IgG antibody level than the control groups. Conclusion Administration of chitosan nanoparticles can be a suitable method to induce more appropriate immune responses against low inherent immunogenic tuberculosis proteins through intranasal routs. © 2016 Informa UK Limited, trading as Taylor & Francis Group.

Item Type: Article
Additional Information: Cited By :8 Export Date: 16 February 2020 Correspondence Address: Amini, Y.; Mashhad Medical UniversityIran; email: aminiy901@mums.ac.ir
Uncontrolled Keywords: Chitosan cytokines ESAT-6 immune responses Mycobacterium tuberculosis Antigens Biodegradable polymers Chitin Nanoparticles Proteins Chitosan nanoparticles Immune response Intranasal immunizations Physio-chemical properties Secreted antigenic target Immune system chitosan nanoparticle early secretory antigenic target 6 gamma interferon immunoglobulin G interleukin 4 nanocarrier bacterial antigen bacterial protein bacterium antibody ESAT-6 protein, Mycobacterium tuberculosis IFNG protein, mouse animal cell animal experiment animal model antibody blood level antibody response Article blood sampling comparative study controlled study cytokine release enzyme linked immunosorbent assay female humoral immunity immunization in vitro study mouse nanoencapsulation nanofabrication nanopharmaceutics nonhuman particle size physical chemistry protein blood level spleen cell tuberculosis zeta potential animal Bagg albino mouse blood drug delivery system immunology intranasal drug administration procedures Administration, Intranasal Animals Antibodies, Bacterial Antigens, Bacterial Bacterial Proteins Drug Delivery Systems Interferon-gamma Interleukin-4 Mice Mice, Inbred BALB C
Subjects: WQ Obstetrics
QW Microbiology and Immunology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib3 lib3
Date Deposited: 12 May 2020 07:26
Last Modified: 12 May 2020 07:26
URI: http://eprints.mums.ac.ir/id/eprint/16744

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