Evaluation of GeneXpert MTB/RIF for determination of rifampicin resistance among new tuberculosis cases in west and northwest Iran

Atashi, S. and Izadi, B. and Jalilian, S. and Madani, S. H. and Farahani, A. and Mohajeri, P. (2017) Evaluation of GeneXpert MTB/RIF for determination of rifampicin resistance among new tuberculosis cases in west and northwest Iran. New Microbes and New Infections, 19. pp. 117-120.

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Abstract

Despite a Mycobacterium tuberculosis control programme and anti-tuberculosis drugs, drug-resistant tuberculosis (DR-TB) is one of the most serious public health issues worldwide. Rapid laboratory diagnosis of M. tuberculosis is needed for the diagnosis of multidrug-resistant (MDR) TB and to find the optimal treatment protocol. The purpose of this study was to detect resistance to rifampicin in new cases of TB using the GeneXpert MTB/RIF (M. tuberculosis/rifampicin) assay and the standard proportional method in west and northwest Iran. In this descriptive cross-sectional study, sputum samples were enrolled and screened for M. tuberculosis using Ziehl–Neelsen stain and mycobacterial culture. Samples from individuals with smear-positive TB were cultured on Lowenstein–Jensen medium; afterwards, the presence of resistance to rifampicin was examined by the GeneXpert MTB/RIF and standard proportional methods. A total of 400 new cases of suspected TB were collected, 162 (40.5) of which were smear- and culture-positive for M. tuberculosis. The frequencies of rifampicin resistance in new smear-positive TB cases were 3.1 and 4.3 for GeneXpert and standard proportional method, respectively. Sensitivity and specificity of GeneXpert were 71 and 100, respectively, compared with the proportional method. GeneXpert can be a quick and helpful method for the diagnosis of rifampicin-resistant TB in regions with high rates of DR-TB or MDR-TB. GeneXpert MTB-RIF assay must be used as an early diagnostic method whose results must be confirmed by the standard proportional method. The GeneXpert and proportional methods complement but do not replace each other. © 2017 The Authors

Item Type: Article
Additional Information: Cited By :5 Export Date: 16 February 2020 Correspondence Address: Mohajeri, P.; Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Shirudi Shahid Blvd, Daneshgah Street, Iran; email: pmohajeri@yahoo.com
Uncontrolled Keywords: GeneXpert MTB/RIF assay multidrug resistance Mycobacterium tuberculosis sensitivity specificity isoniazid rifampicin adolescent adult aged antibiotic resistance antibiotic sensitivity Article bacterium detection bacterium isolate cross-sectional study diagnostic test female human Iran major clinical study male Mycobacterium tuberculosis test kit polymerase chain reaction priority journal screening sensitivity and specificity sputum analysis tuberculosis
Subjects: WF Respiratory System
QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib3 lib3
Date Deposited: 18 May 2020 04:29
Last Modified: 18 May 2020 04:29
URI: http://eprints.mums.ac.ir/id/eprint/16756

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