Quantitative assessment of wilms tumor 1 expression by real-time quantitative polymerase chain reaction in patients with acute myeloblastic leukemia

Ayatollahi, H. and Sadeghian, M. H. and Naderi, M. and Jafarian, A. H. and Shams, S. F. and Motamedirad, N. and Sheikhi, M. and Bahrami, A. and Shakeri, S. (2017) Quantitative assessment of wilms tumor 1 expression by real-time quantitative polymerase chain reaction in patients with acute myeloblastic leukemia. Journal of Research in Medical Sciences, 22 (1).

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Abstract

Background: The Wilms tumor 1 (WT1) gene is originally defined as a tumor suppressor gene and a transcription factor that overexpressed in leukemic cells. It is highly expressed in more than 80 of acute myeloid leukemia (AML) patients, both in bone marrow (BM) and in peripheral blood (PB), and it is used as a powerful and independent marker of minimal residual disease (MRD);we have determined the expression levels of the WT1 by real-time quantitative polymerase chain reaction (RQ-PCR) in PB and BM in 126 newly diagnosed AML patients. Materials and Methods: This study was done in molecular pathology and cancer research center from April 2014 to June 2015, RQ-PCR method was used to determine the WT1 gene expression in BM and/or PB samples from 126 patients of AML, we cloned both WT1 and ABL genes for creating a standard curve, and we calculate copy number of WT1 genes in patients. Results: A total of 126 AML patients consist of 70 males (55.6) and 56 females (44.4), with a median age of 26 years; 104 (81) patients out of 126 show overexpression of WT1 gene. We also concomitant monitoring of fusion transcripts (PML RARa, AML1-ETO, MLL-MLL, CBFb-MYH11, or DEK-CAN) in our patients, the AML1-ETO group showing remarkably low levels of WT1 compared with other fusion transcript and the CBFB-MYH11 showing high levels of WT1. Conclusion: We conclude that WT1 expression by RQ-PCR in AML patients may be employed as an independent tool to detect MRD in the majority of normal karyotype AML patients. © 2017 Journal of Research in Medical Sciences.

Item Type: Article
Additional Information: Cited By :2 Export Date: 16 February 2020 Correspondence Address: Shakeri, S.; Cancer Molecular Pathology Research Center, Faculty of Medicine, Mashhad University of Medical SciencesIran; email: se.shakeri@yahoo.com
Uncontrolled Keywords: Acute myeloid leukemia Minimal residual disease Real-time quantitative polymerase chain reaction Wilms tumor gene CD14 antigen CD64 antigen esterase microsomal aminopeptidase myeloperoxidase stem cell factor receptor adolescent adult aged Article cell structure child chromosome analysis controlled study female gene dosage gene expression gene overexpression human major clinical study male molecular cloning real time polymerase chain reaction reverse transcription polymerase chain reaction spectrophotometry tumor suppressor gene
Subjects: WH Hemic and Lymphatic System
QU Biochemistry
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib3 lib3
Date Deposited: 18 May 2020 06:56
Last Modified: 18 May 2020 06:56
URI: http://eprints.mums.ac.ir/id/eprint/16758

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